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Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter

Author

Listed:
  • Guangjie Cui

    (University of Michigan)

  • Yunbo Liu

    (University of Michigan)

  • Di Zu

    (University of Michigan)

  • Xintao Zhao

    (University of Michigan)

  • Zhijia Zhang

    (University of Michigan)

  • Do Young Kim

    (University of Michigan)

  • Pramith Senaratne

    (University of Michigan)

  • Aaron Fox

    (University of Michigan)

  • David Sept

    (University of Michigan)

  • Younggeun Park

    (University of Michigan)

  • Somin Eunice Lee

    (University of Michigan)

Abstract

Fundamental to all living organisms and living soft matter are emergent processes in which the reorganization of individual constituents at the nanoscale drives group-level movements and shape changes at the macroscale over time. However, light-induced degradation of fluorophores, photobleaching, is a significant problem in extended bioimaging in life science. Here, we report opening a long-time investigation window by nonbleaching phase intensity nanoscope: PINE. We accomplish phase-intensity separation such that nanoprobe distributions are distinguished by an integrated phase-intensity multilayer thin film (polyvinyl alcohol/liquid crystal). We overcame a physical limit to resolve sub-10 nm cellular architectures, and achieve the first dynamic imaging of nanoscopic reorganization over 250 h using PINE. We discover nanoscopic rearrangements synchronized with the emergence of group-level movements and shape changes at the macroscale according to a set of interaction rules with importance in cellular and soft matter reorganization, self-organization, and pattern formation.

Suggested Citation

  • Guangjie Cui & Yunbo Liu & Di Zu & Xintao Zhao & Zhijia Zhang & Do Young Kim & Pramith Senaratne & Aaron Fox & David Sept & Younggeun Park & Somin Eunice Lee, 2023. "Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39624-w
    DOI: 10.1038/s41467-023-39624-w
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    References listed on IDEAS

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