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Rapid and reversible optical switching of cell membrane area by an amphiphilic azobenzene

Author

Listed:
  • Fabian Höglsperger

    (University of Münster
    University of Münster)

  • Bart E. Vos

    (University of Göttingen)

  • Arne D. Hofemeier

    (University of Göttingen)

  • Maximilian D. Seyfried

    (University of Münster
    University of Münster)

  • Bastian Stövesand

    (University of Münster
    University of Münster)

  • Azadeh Alavizargar

    (University of Münster)

  • Leon Topp

    (University of Münster)

  • Andreas Heuer

    (University of Münster
    University of Münster)

  • Timo Betz

    (University of Göttingen)

  • Bart Jan Ravoo

    (University of Münster
    University of Münster)

Abstract

Cellular membrane area is a key parameter for any living cell that is tightly regulated to avoid membrane damage. Changes in area-to-volume ratio are known to be critical for cell shape, but are mostly investigated by changing the cell volume via osmotic shocks. In turn, many important questions relating to cellular shape, membrane tension homeostasis and local membrane area cannot be easily addressed because experimental tools for controlled modulation of cell membrane area are lacking. Here we show that photoswitching an amphiphilic azobenzene can trigger its intercalation into the plasma membrane of various mammalian cells ranging from erythrocytes to myoblasts and cancer cells. The photoisomerization leads to a rapid (250-500 ms) and highly reversible membrane area change (ca 2 % for erythrocytes) that triggers a dramatic shape modulation of living cells.

Suggested Citation

  • Fabian Höglsperger & Bart E. Vos & Arne D. Hofemeier & Maximilian D. Seyfried & Bastian Stövesand & Azadeh Alavizargar & Leon Topp & Andreas Heuer & Timo Betz & Bart Jan Ravoo, 2023. "Rapid and reversible optical switching of cell membrane area by an amphiphilic azobenzene," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39032-0
    DOI: 10.1038/s41467-023-39032-0
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    References listed on IDEAS

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    1. Kazuya Tsujita & Reiko Satow & Shinobu Asada & Yoshikazu Nakamura & Luis Arnes & Keisuke Sako & Yasuyuki Fujita & Kiyoko Fukami & Toshiki Itoh, 2021. "Homeostatic membrane tension constrains cancer cell dissemination by counteracting BAR protein assembly," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Harvey T. McMahon & Jennifer L. Gallop, 2005. "Membrane curvature and mechanisms of dynamic cell membrane remodelling," Nature, Nature, vol. 438(7068), pages 590-596, December.
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    Cited by:

    1. Carlos A. Z. Bassetto & Juergen Pfeffermann & Rohit Yadav & Simon Strassgschwandtner & Toma Glasnov & Francisco Bezanilla & Peter Pohl, 2024. "Photolipid excitation triggers depolarizing optocapacitive currents and action potentials," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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