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Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles

Author

Listed:
  • Ewa Sitarska

    (European Molecular Biology Laboratory
    EMBL and Heidelberg University)

  • Silvia Dias Almeida

    (European Molecular Biology Laboratory
    German Cancer Research Center (DKFZ))

  • Marianne Sandvold Beckwith

    (European Molecular Biology Laboratory)

  • Julian Stopp

    (Institute of Science and Technology Austria)

  • Jakub Czuchnowski

    (European Molecular Biology Laboratory)

  • Marc Siggel

    (European Molecular Biology Laboratory
    Centre for Structural Systems Biology)

  • Rita Roessner

    (European Molecular Biology Laboratory
    Centre for Structural Systems Biology)

  • Aline Tschanz

    (European Molecular Biology Laboratory
    EMBL and Heidelberg University)

  • Christer Ejsing

    (European Molecular Biology Laboratory
    University of Southern Denmark)

  • Yannick Schwab

    (European Molecular Biology Laboratory)

  • Jan Kosinski

    (European Molecular Biology Laboratory
    Centre for Structural Systems Biology
    European Molecular Biology Laboratory)

  • Michael Sixt

    (Institute of Science and Technology Austria)

  • Anna Kreshuk

    (European Molecular Biology Laboratory)

  • Anna Erzberger

    (European Molecular Biology Laboratory)

  • Alba Diz-Muñoz

    (European Molecular Biology Laboratory)

Abstract

To navigate through diverse tissues, migrating cells must balance persistent self-propelled motion with adaptive behaviors to circumvent obstacles. We identify a curvature-sensing mechanism underlying obstacle evasion in immune-like cells. Specifically, we propose that actin polymerization at the advancing edge of migrating cells is inhibited by the curvature-sensitive BAR domain protein Snx33 in regions with inward plasma membrane curvature. The genetic perturbation of this machinery reduces the cells’ capacity to evade obstructions combined with faster and more persistent cell migration in obstacle-free environments. Our results show how cells can read out their surface topography and utilize actin and plasma membrane biophysics to interpret their environment, allowing them to adaptively decide if they should move ahead or turn away. On the basis of our findings, we propose that the natural diversity of BAR domain proteins may allow cells to tune their curvature sensing machinery to match the shape characteristics in their environment.

Suggested Citation

  • Ewa Sitarska & Silvia Dias Almeida & Marianne Sandvold Beckwith & Julian Stopp & Jakub Czuchnowski & Marc Siggel & Rita Roessner & Aline Tschanz & Christer Ejsing & Yannick Schwab & Jan Kosinski & Mic, 2023. "Sensing their plasma membrane curvature allows migrating cells to circumvent obstacles," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41173-1
    DOI: 10.1038/s41467-023-41173-1
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    References listed on IDEAS

    as
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