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Two RhoGEF isoforms with distinct localisation control furrow position during asymmetric cell division

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  • Emilie Montembault

    (University of Bordeaux, Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit
    CNRS, UMR5095, University of Bordeaux, Institut de Biologie et Génétique Cellulaire, 1 rue Camille Saint-Saëns)

  • Irène Deduyer

    (University of Bordeaux, Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit
    CNRS, UMR5095, University of Bordeaux, Institut de Biologie et Génétique Cellulaire, 1 rue Camille Saint-Saëns)

  • Marie-Charlotte Claverie

    (University of Bordeaux, Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit
    CNRS, UMR5095, University of Bordeaux, Institut de Biologie et Génétique Cellulaire, 1 rue Camille Saint-Saëns)

  • Lou Bouit

    (University of Bordeaux, Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit
    University of Bordeaux)

  • Nicolas J. Tourasse

    (University of Bordeaux, INSERM, U1212, Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit)

  • Denis Dupuy

    (University of Bordeaux, INSERM, U1212, Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit)

  • Derek McCusker

    (University of Bordeaux, Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit
    CNRS, UMR5095, University of Bordeaux, Institut de Biologie et Génétique Cellulaire, 1 rue Camille Saint-Saëns)

  • Anne Royou

    (University of Bordeaux, Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit
    CNRS, UMR5095, University of Bordeaux, Institut de Biologie et Génétique Cellulaire, 1 rue Camille Saint-Saëns)

Abstract

Cytokinesis partitions cellular content between daughter cells. It relies on the formation of an acto-myosin contractile ring, whose constriction induces the ingression of the cleavage furrow between the segregated chromatids. Rho1 GTPase and its RhoGEF (Pbl) are essential for this process. However, how Rho1 is regulated to sustain furrow ingression while maintaining correct furrow position remains poorly defined. Here, we show that during asymmetric division of Drosophila neuroblasts, Rho1 is controlled by two Pbl isoforms with distinct localisation. Spindle midzone- and furrow-enriched Pbl-A focuses Rho1 at the furrow to sustain efficient ingression, while Pbl-B pan-plasma membrane localization promotes the broadening of Rho1 activity and the subsequent enrichment of myosin on the entire cortex. This enlarged zone of Rho1 activity is critical to adjust furrow position, thereby preserving correct daughter cell size asymmetry. Our work highlights how the use of isoforms with distinct localisation makes an essential process more robust.

Suggested Citation

  • Emilie Montembault & Irène Deduyer & Marie-Charlotte Claverie & Lou Bouit & Nicolas J. Tourasse & Denis Dupuy & Derek McCusker & Anne Royou, 2023. "Two RhoGEF isoforms with distinct localisation control furrow position during asymmetric cell division," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38912-9
    DOI: 10.1038/s41467-023-38912-9
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    References listed on IDEAS

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    1. Michaela Roth & Chantal Roubinet & Niklas Iffländer & Alexia Ferrand & Clemens Cabernard, 2015. "Asymmetrically dividing Drosophila neuroblasts utilize two spatially and temporally independent cytokinesis pathways," Nature Communications, Nature, vol. 6(1), pages 1-14, May.
    2. Kei Yamamoto & Haruko Miura & Motohiko Ishida & Yusuke Mii & Noriyuki Kinoshita & Shinji Takada & Naoto Ueno & Satoshi Sawai & Yohei Kondo & Kazuhiro Aoki, 2021. "Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    3. Jakub Sedzinski & Maté Biro & Annelie Oswald & Jean-Yves Tinevez & Guillaume Salbreux & Ewa Paluch, 2011. "Polar actomyosin contractility destabilizes the position of the cytokinetic furrow," Nature, Nature, vol. 476(7361), pages 462-466, August.
    4. Andrew W. Senior & Richard Evans & John Jumper & James Kirkpatrick & Laurent Sifre & Tim Green & Chongli Qin & Augustin Žídek & Alexander W. R. Nelson & Alex Bridgland & Hugo Penedones & Stig Petersen, 2020. "Improved protein structure prediction using potentials from deep learning," Nature, Nature, vol. 577(7792), pages 706-710, January.
    5. Clemens Cabernard & Kenneth E. Prehoda & Chris Q. Doe, 2010. "A spindle-independent cleavage furrow positioning pathway," Nature, Nature, vol. 467(7311), pages 91-94, September.
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