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Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus

Author

Listed:
  • Pengcheng Shang

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh)

  • Joshua D. Simpson

    (Université catholique de Louvain)

  • Gwen M. Taylor

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh)

  • Danica M. Sutherland

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh)

  • Olivia L. Welsh

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh)

  • Pavithra Aravamudhan

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh)

  • Rita Dos Santos Natividade

    (Université catholique de Louvain)

  • Kristina Schwab

    (University of Pittsburgh School of Medicine)

  • Joshua J. Michel

    (University of Pittsburgh School of Medicine)

  • Amanda C. Poholek

    (University of Pittsburgh School of Medicine
    University of Pittsburgh School of Medicine)

  • Yijen Wu

    (University of Pittsburgh School of Medicine)

  • Dhivyaa Rajasundaram

    (University of Pittsburgh School of Medicine)

  • Melanie Koehler

    (Leibniz Institute for Food Systems Biology at the Technical University Munich)

  • David Alsteens

    (Université catholique de Louvain
    WEL Research Institute)

  • Terence S. Dermody

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh
    University of Pittsburgh School of Medicine)

Abstract

Mammalian orthoreovirus (reovirus) infects most mammals and is associated with celiac disease in humans. In mice, reovirus infects the intestine and disseminates systemically to cause serotype-specific patterns of disease in the brain. To identify receptors conferring reovirus serotype-dependent neuropathogenesis, we conducted a genome-wide CRISPRa screen and identified paired immunoglobulin-like receptor B (PirB) as a receptor candidate. Ectopic expression of PirB allowed reovirus binding and infection. PirB extracelluar D3D4 region is required for reovirus attachment and infectivity. Reovirus binds to PirB with nM affinity as determined by single molecule force spectroscopy. Efficient reovirus endocytosis requires PirB signaling motifs. In inoculated mice, PirB is required for maximal replication in the brain and full neuropathogenicity of neurotropic serotype 3 (T3) reovirus. In primary cortical neurons, PirB expression contributes to T3 reovirus infectivity. Thus, PirB is an entry receptor for reovirus and contributes to T3 reovirus replication and pathogenesis in the murine brain.

Suggested Citation

  • Pengcheng Shang & Joshua D. Simpson & Gwen M. Taylor & Danica M. Sutherland & Olivia L. Welsh & Pavithra Aravamudhan & Rita Dos Santos Natividade & Kristina Schwab & Joshua J. Michel & Amanda C. Pohol, 2023. "Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38327-6
    DOI: 10.1038/s41467-023-38327-6
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    References listed on IDEAS

    as
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