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Glycan-mediated enhancement of reovirus receptor binding

Author

Listed:
  • Melanie Koehler

    (Université catholique de Louvain)

  • Pavithra Aravamudhan

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh)

  • Camila Guzman-Cardozo

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh)

  • Andra C. Dumitru

    (Université catholique de Louvain)

  • Jinsung Yang

    (Université catholique de Louvain)

  • Serena Gargiulo

    (Université catholique de Louvain)

  • Patrice Soumillion

    (Université catholique de Louvain)

  • Terence S. Dermody

    (University of Pittsburgh School of Medicine
    UPMC Children’s Hospital of Pittsburgh
    University of Pittsburgh School of Medicine)

  • David Alsteens

    (Université catholique de Louvain)

Abstract

Viral infection is an intricate process that requires the concerted action of both viral and host cell components. Entry of viruses into cells is initiated by interactions between viral proteins and their cell surface receptors. Despite recent progress, the molecular mechanisms underlying the multistep reovirus entry process are poorly understood. Using atomic force microscopy, we investigated how the reovirus σ1 attachment protein binds to both α-linked sialic acid (α-SA) and JAM-A cell-surface receptors. We discovered that initial σ1 binding to α-SA favors a strong multivalent anchorage to JAM-A. The enhanced JAM-A binding by virions following α-SA engagement is comparable to JAM-A binding by infectious subvirion particles (ISVPs) in the absence of α-SA. Since ISVPs have an extended σ1 conformer, this finding suggests that α-SA binding triggers a conformational change in σ1. These results provide new insights into the function of viral attachment proteins in the initiation of infection and open new avenues for the use of reoviruses as oncolytic agents.

Suggested Citation

  • Melanie Koehler & Pavithra Aravamudhan & Camila Guzman-Cardozo & Andra C. Dumitru & Jinsung Yang & Serena Gargiulo & Patrice Soumillion & Terence S. Dermody & David Alsteens, 2019. "Glycan-mediated enhancement of reovirus receptor binding," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12411-2
    DOI: 10.1038/s41467-019-12411-2
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    Cited by:

    1. Pengcheng Shang & Joshua D. Simpson & Gwen M. Taylor & Danica M. Sutherland & Olivia L. Welsh & Pavithra Aravamudhan & Rita Dos Santos Natividade & Kristina Schwab & Joshua J. Michel & Amanda C. Pohol, 2023. "Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Simon J. L. Petitjean & Wenzhang Chen & Melanie Koehler & Ravikumar Jimmidi & Jinsung Yang & Danahe Mohammed & Blinera Juniku & Megan L. Stanifer & Steeve Boulant & Stéphane P. Vincent & David Alsteen, 2022. "Multivalent 9-O-Acetylated-sialic acid glycoclusters as potent inhibitors for SARS-CoV-2 infection," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Melanie Koehler & Ankita Ray & Rodrigo A. Moreira & Blinera Juniku & Adolfo B. Poma & David Alsteens, 2021. "Molecular insights into receptor binding energetics and neutralization of SARS-CoV-2 variants," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

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