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HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing

Author

Listed:
  • Chao Yang

    (Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Zhenzhen Ma

    (Nankai University)

  • Keshan Wang

    (Huazhong University of Science and Technology)

  • Xingxiao Dong

    (Dalian Polytechnic University)

  • Meiyu Huang

    (Guangxi Normal University)

  • Yaqiu Li

    (Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Xiagu Zhu

    (Tianjin University of Science and Technology)

  • Ju Li

    (Tianjin Normal University)

  • Zhihui Cheng

    (Nankai University)

  • Changhao Bi

    (Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Xueli Zhang

    (Chinese Academy of Sciences
    Chinese Academy of Sciences)

Abstract

C-to-G base editors have been successfully constructed recently, but limited work has been done on concurrent C-to-G and A-to-G base editing. In addition, there is also limited data on how chromatin-associated factors affect the base editing. Here, we test a series of chromatin-associated factors, and chromosomal protein HMGN1 was found to enhance the efficiency of both C-to-G and A-to-G base editing. By fusing HMGN1, GBE and ABE to Cas9, we develop a CRISPR-based dual-function A-to-G and C-to-G base editor (GGBE) which is capable of converting simultaneous A and C to G conversion with substantial editing efficiency. Accordingly, the HMGN1 role shown in this work and the resulting GGBE tool further broaden the genome manipulation capacity of CRISPR-directed base editors.

Suggested Citation

  • Chao Yang & Zhenzhen Ma & Keshan Wang & Xingxiao Dong & Meiyu Huang & Yaqiu Li & Xiagu Zhu & Ju Li & Zhihui Cheng & Changhao Bi & Xueli Zhang, 2023. "HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38193-2
    DOI: 10.1038/s41467-023-38193-2
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