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Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats

Author

Listed:
  • Zeyu Lu

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Lingtian Zhang

    (Cofco Jia Jia Kang Food Co., Ltd., Songyuan City 131500, China)

  • Qing Mu

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Junyang Liu

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Yu Chen

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Haoyuan Wang

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Yanjun Zhang

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Rui Su

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Ruijun Wang

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Zhiying Wang

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Qi Lv

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Zhihong Liu

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

  • Jiasen Liu

    (Institute of Animal Husbandry, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China)

  • Yunhua Li

    (Institute of Animal Husbandry, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China)

  • Yanhong Zhao

    (Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China
    Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot 010018, China
    College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China)

Abstract

Due to recent innovations in gene editing technology, great progress has been made in livestock breeding, with researchers rearing gene-edited pigs, cattle, sheep, and other livestock. Gene-editing technology involves knocking in, knocking out, deleting, inhibiting, activating, or replacing specific bases of DNA or RNA sequences at the genome level for accurate modification, and such processes can edit genes at a fixed point without needing DNA templates. In recent years, although clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system-mediated gene-editing technology has been widely used in research into the genetic breeding of animals, the system’s efficiency at inserting foreign genes is not high enough, and there are certain off-target effects; thus, it is not appropriate for use in the genome editing of large livestock such as cashmere goats. In this study, the development status, associated challenges, application prospects, and future prospects of CRISPR/Cas9-mediated precision gene-editing technology for use in livestock breeding were reviewed to provide a theoretical reference for livestock gene function analysis, genetic improvement, and livestock breeding that account for characteristics of local economies.

Suggested Citation

  • Zeyu Lu & Lingtian Zhang & Qing Mu & Junyang Liu & Yu Chen & Haoyuan Wang & Yanjun Zhang & Rui Su & Ruijun Wang & Zhiying Wang & Qi Lv & Zhihong Liu & Jiasen Liu & Yunhua Li & Yanhong Zhao, 2024. "Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats," Agriculture, MDPI, vol. 14(3), pages 1-17, March.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:3:p:487-:d:1358721
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    References listed on IDEAS

    as
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    Full references (including those not matched with items on IDEAS)

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