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DNA-binding mechanism and evolution of replication protein A

Author

Listed:
  • Clément Madru

    (Institut Pasteur, Université Paris Cité, CNRS)

  • Markel Martínez-Carranza

    (Institut Pasteur, Université Paris Cité, CNRS)

  • Sébastien Laurent

    (Univ Brest, Ifremer, CNRS, Biologie et Ecologie des Ecoystèmes marins profonds (BEEP))

  • Alessandra C. Alberti

    (Institut Pasteur, Université Paris Cité, CNRS)

  • Maelenn Chevreuil

    (C2RT, Institut Pasteur, Université Paris Cité, CNRS)

  • Bertrand Raynal

    (C2RT, Institut Pasteur, Université Paris Cité, CNRS)

  • Ahmed Haouz

    (C2RT, Institut Pasteur, Université Paris Cité, CNRS)

  • Rémy A. Meur

    (C2RT, Institut Pasteur, Université Paris Cité, CNRS)

  • Marc Delarue

    (Institut Pasteur, Université Paris Cité, CNRS)

  • Ghislaine Henneke

    (Univ Brest, Ifremer, CNRS, Biologie et Ecologie des Ecoystèmes marins profonds (BEEP))

  • Didier Flament

    (Univ Brest, Ifremer, CNRS, Biologie et Ecologie des Ecoystèmes marins profonds (BEEP))

  • Mart Krupovic

    (Institut Pasteur, Université Paris Cité, CNRS)

  • Pierre Legrand

    (Institut Pasteur, Université Paris Cité, CNRS
    Synchrotron SOLEIL, HelioBio group, L’Orme des Merisiers)

  • Ludovic Sauguet

    (Institut Pasteur, Université Paris Cité, CNRS)

Abstract

Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in Archaea, the third domain of life. By using an integrative structural, biochemical and biophysical approach, we extensively characterize RPA from Pyrococcus abyssi in the presence and absence of DNA. The obtained X-ray and cryo-EM structures reveal that the trimerization core and interactions promoting RPA clustering on ssDNA are shared between archaea and eukaryotes. However, we also identified a helical domain named AROD (Acidic Rpa1 OB-binding Domain), and showed that, in Archaea, RPA forms an unanticipated tetrameric supercomplex in the absence of DNA. The four RPA molecules clustered within the tetramer could efficiently coat and protect stretches of ssDNA created by the advancing replisome. Finally, our results provide insights into the evolution of this primordial replication factor in eukaryotes.

Suggested Citation

  • Clément Madru & Markel Martínez-Carranza & Sébastien Laurent & Alessandra C. Alberti & Maelenn Chevreuil & Bertrand Raynal & Ahmed Haouz & Rémy A. Meur & Marc Delarue & Ghislaine Henneke & Didier Flam, 2023. "DNA-binding mechanism and evolution of replication protein A," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38048-w
    DOI: 10.1038/s41467-023-38048-w
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    Cited by:

    1. Markel Martínez-Carranza & Léa Vialle & Clément Madru & Florence Cordier & Ayten Dizkirici Tekpinar & Ahmed Haouz & Pierre Legrand & Rémy A. Meur & Patrick England & Rémi Dulermo & J. Iñaki Guijarro &, 2024. "Communication between DNA polymerases and Replication Protein A within the archaeal replisome," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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