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Communication between DNA polymerases and Replication Protein A within the archaeal replisome

Author

Listed:
  • Markel Martínez-Carranza

    (Université Paris Cité, CNRS UMR 3528)

  • Léa Vialle

    (Univ Brest, Ifremer, CNRS, Biologie et Ecologie des Ecoystèmes marins profonds (BEEP))

  • Clément Madru

    (Université Paris Cité, CNRS UMR 3528)

  • Florence Cordier

    (Université Paris Cité, CNRS UMR 3528
    Université Paris Cité, CNRS UMR 3528)

  • Ayten Dizkirici Tekpinar

    (Université Paris Cité, CNRS UMR 3528
    Van Yüzüncü Yil University)

  • Ahmed Haouz

    (Université Paris Cité, CNRS UMR 3528)

  • Pierre Legrand

    (L’Orme des Merisiers)

  • Rémy A. Meur

    (Université Paris Cité, CNRS UMR 3528)

  • Patrick England

    (Université Paris Cité, CNRS UMR 3528)

  • Rémi Dulermo

    (Univ Brest, Ifremer, CNRS, Biologie et Ecologie des Ecoystèmes marins profonds (BEEP))

  • J. Iñaki Guijarro

    (Université Paris Cité, CNRS UMR 3528)

  • Ghislaine Henneke

    (Univ Brest, Ifremer, CNRS, Biologie et Ecologie des Ecoystèmes marins profonds (BEEP))

  • Ludovic Sauguet

    (Université Paris Cité, CNRS UMR 3528)

Abstract

Replication Protein A (RPA) plays a pivotal role in DNA replication by coating and protecting exposed single-stranded DNA, and acting as a molecular hub that recruits additional replication factors. We demonstrate that archaeal RPA hosts a winged-helix domain (WH) that interacts with two key actors of the replisome: the DNA primase (PriSL) and the replicative DNA polymerase (PolD). Using an integrative structural biology approach, combining nuclear magnetic resonance, X-ray crystallography and cryo-electron microscopy, we unveil how RPA interacts with PriSL and PolD through two distinct surfaces of the WH domain: an evolutionarily conserved interface and a novel binding site. Finally, RPA is shown to stimulate the activity of PriSL in a WH-dependent manner. This study provides a molecular understanding of the WH-mediated regulatory activity in central replication factors such as RPA, which regulate genome maintenance in Archaea and Eukaryotes.

Suggested Citation

  • Markel Martínez-Carranza & Léa Vialle & Clément Madru & Florence Cordier & Ayten Dizkirici Tekpinar & Ahmed Haouz & Pierre Legrand & Rémy A. Meur & Patrick England & Rémi Dulermo & J. Iñaki Guijarro &, 2024. "Communication between DNA polymerases and Replication Protein A within the archaeal replisome," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-55365-w
    DOI: 10.1038/s41467-024-55365-w
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