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Trumpet is an operating system for simple and robust cell-free biocomputing

Author

Listed:
  • Judee A. Sharon

    (University of Minnesota, Twin Cities)

  • Chelsea Dasrath

    (University of Minnesota, Twin Cities)

  • Aiden Fujiwara

    (University of Minnesota, Twin Cities)

  • Alessandro Snyder

    (University of Minnesota, Twin Cities)

  • Mace Blank

    (University of Minnesota, Twin Cities)

  • Sam O’Brien

    (University of Minnesota, Twin Cities)

  • Lauren M. Aufdembrink

    (University of Minnesota, Twin Cities)

  • Aaron E. Engelhart

    (University of Minnesota, Twin Cities)

  • Katarzyna P. Adamala

    (University of Minnesota, Twin Cities)

Abstract

Biological computation is becoming a viable and fast-growing alternative to traditional electronic computing. Here we present a biocomputing technology called Trumpet: Transcriptional RNA Universal Multi-Purpose GatE PlaTform. Trumpet combines the simplicity and robustness of the simplest in vitro biocomputing methods, adding signal amplification and programmability, while avoiding common shortcomings of live cell-based biocomputing solutions. We have demonstrated the use of Trumpet to build all universal Boolean logic gates. We have also built a web-based platform for designing Trumpet gates and created a primitive processor by networking several gates as a proof-of-principle for future development. The Trumpet offers a change of paradigm in biocomputing, providing an efficient and easily programmable biological logic gate operating system.

Suggested Citation

  • Judee A. Sharon & Chelsea Dasrath & Aiden Fujiwara & Alessandro Snyder & Mace Blank & Sam O’Brien & Lauren M. Aufdembrink & Aaron E. Engelhart & Katarzyna P. Adamala, 2023. "Trumpet is an operating system for simple and robust cell-free biocomputing," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37752-x
    DOI: 10.1038/s41467-023-37752-x
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    References listed on IDEAS

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