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Microridge-like structures anchor motile cilia

Author

Listed:
  • Takayuki Yasunaga

    (University of Freiburg)

  • Johannes Wiegel

    (University of Freiburg)

  • Max D. Bergen

    (University of Freiburg)

  • Martin Helmstädter

    (University of Freiburg)

  • Daniel Epting

    (University of Freiburg)

  • Andrea Paolini

    (University of Freiburg
    University of Freiburg)

  • Özgün Çiçek

    (University of Freiburg
    BIOSS Centre for Biological Signalling Studies, University of Freiburg)

  • Gerald Radziwill

    (BIOSS Centre for Biological Signalling Studies, University of Freiburg
    CIBSS Centre for Integrative Biological Signalling Studies, University of Freiburg)

  • Christina Engel

    (University of Freiburg)

  • Thomas Brox

    (University of Freiburg
    BIOSS Centre for Biological Signalling Studies, University of Freiburg
    CIBSS Centre for Integrative Biological Signalling Studies, University of Freiburg)

  • Olaf Ronneberger

    (University of Freiburg
    BIOSS Centre for Biological Signalling Studies, University of Freiburg)

  • Peter Walentek

    (University of Freiburg
    CIBSS Centre for Integrative Biological Signalling Studies, University of Freiburg)

  • Maximilian H. Ulbrich

    (University of Freiburg
    BIOSS Centre for Biological Signalling Studies, University of Freiburg)

  • Gerd Walz

    (University of Freiburg
    BIOSS Centre for Biological Signalling Studies, University of Freiburg
    CIBSS Centre for Integrative Biological Signalling Studies, University of Freiburg)

Abstract

Several tissues contain cells with multiple motile cilia that generate a fluid or particle flow to support development and organ functions; defective motility causes human disease. Developmental cues orient motile cilia, but how cilia are locked into their final position to maintain a directional flow is not understood. Here we find that the actin cytoskeleton is highly dynamic during early development of multiciliated cells (MCCs). While apical actin bundles become increasingly more static, subapical actin filaments are nucleated from the distal tip of ciliary rootlets. Anchorage of these subapical actin filaments requires the presence of microridge-like structures formed during MCC development, and the activity of Nonmuscle Myosin II. Optogenetic manipulation of Ezrin, a core component of the microridge actin-anchoring complex, or inhibition of Myosin Light Chain Kinase interfere with rootlet anchorage and orientation. These observations identify microridge-like structures as an essential component of basal body rootlet anchoring in MCCs.

Suggested Citation

  • Takayuki Yasunaga & Johannes Wiegel & Max D. Bergen & Martin Helmstädter & Daniel Epting & Andrea Paolini & Özgün Çiçek & Gerald Radziwill & Christina Engel & Thomas Brox & Olaf Ronneberger & Peter Wa, 2022. "Microridge-like structures anchor motile cilia," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29741-3
    DOI: 10.1038/s41467-022-29741-3
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    References listed on IDEAS

    as
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