IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-37055-1.html
   My bibliography  Save this article

Microbiota alters the metabolome in an age- and sex- dependent manner in mice

Author

Listed:
  • Kirsty Brown

    (University of Calgary)

  • Carolyn A. Thomson

    (University of Calgary)

  • Soren Wacker

    (University of Calgary)

  • Marija Drikic

    (University of Calgary)

  • Ryan Groves

    (University of Calgary)

  • Vina Fan

    (University of Calgary)

  • Ian A. Lewis

    (University of Calgary)

  • Kathy D. McCoy

    (University of Calgary)

Abstract

Commensal bacteria are major contributors to mammalian metabolism. We used liquid chromatography mass spectrometry to study the metabolomes of germ-free, gnotobiotic, and specific-pathogen-free mice, while also evaluating the influence of age and sex on metabolite profiles. Microbiota modified the metabolome of all body sites and accounted for the highest proportion of variation within the gastrointestinal tract. Microbiota and age explained similar amounts of variation the metabolome of urine, serum, and peritoneal fluid, while age was the primary driver of variation in the liver and spleen. Although sex explained the least amount of variation at all sites, it had a significant impact on all sites except the ileum. Collectively, these data illustrate the interplay between microbiota, age, and sex in the metabolic phenotypes of diverse body sites. This provides a framework for interpreting complex metabolic phenotypes and will help guide future studies into the role that the microbiome plays in disease.

Suggested Citation

  • Kirsty Brown & Carolyn A. Thomson & Soren Wacker & Marija Drikic & Ryan Groves & Vina Fan & Ian A. Lewis & Kathy D. McCoy, 2023. "Microbiota alters the metabolome in an age- and sex- dependent manner in mice," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37055-1
    DOI: 10.1038/s41467-023-37055-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-37055-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-37055-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Henrik M. Roager & Tine R. Licht, 2018. "Microbial tryptophan catabolites in health and disease," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    2. Thomas Rydzak & Ryan A. Groves & Ruichuan Zhang & Raied Aburashed & Rajnigandha Pushpker & Maryam Mapar & Ian A. Lewis, 2022. "Metabolic preference assay for rapid diagnosis of bloodstream infections," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Jun Ding & Jian Ji & Zachary Rabow & Tong Shen & Jacob Folz & Christopher R. Brydges & Sili Fan & Xinchen Lu & Sajjan Mehta & Megan R. Showalter & Ying Zhang & Renee Araiza & Lynette R. Bower & K. C. , 2021. "A metabolome atlas of the aging mouse brain," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    4. Robert A. Quinn & Alexey V. Melnik & Alison Vrbanac & Ting Fu & Kathryn A. Patras & Mitchell P. Christy & Zsolt Bodai & Pedro Belda-Ferre & Anupriya Tripathi & Lawton K. Chung & Michael Downes & Ryan , 2020. "Global chemical effects of the microbiome include new bile-acid conjugations," Nature, Nature, vol. 579(7797), pages 123-129, March.
    5. Koen F. Dekkers & Sergi Sayols-Baixeras & Gabriel Baldanzi & Christoph Nowak & Ulf Hammar & Diem Nguyen & Georgios Varotsis & Louise Brunkwall & Nynne Nielsen & Aron C. Eklund & Jacob Bak Holm & H. Bj, 2022. "An online atlas of human plasma metabolite signatures of gut microbiome composition," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Giorgia Renga & Fiorella D’Onofrio & Marilena Pariano & Roberta Galarini & Carolina Barola & Claudia Stincardini & Marina M. Bellet & Helmut Ellemunter & Cornelia Lass-Flörl & Claudio Costantini & Val, 2023. "Bridging of host-microbiota tryptophan partitioning by the serotonin pathway in fungal pneumonia," Nature Communications, Nature, vol. 14(1), pages 1-21, December.
    2. Louise Grahnemo & Maria Nethander & Eivind Coward & Maiken Elvestad Gabrielsen & Satya Sree & Jean-Marc Billod & Klara Sjögren & Lars Engstrand & Koen F. Dekkers & Tove Fall & Arnulf Langhammer & Kris, 2023. "Identification of three bacterial species associated with increased appendicular lean mass: the HUNT study," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    3. Marta G. Novelle, 2021. "Decoding the Role of Gut-Microbiome in the Food Addiction Paradigm," IJERPH, MDPI, vol. 18(13), pages 1-19, June.
    4. Shuling Xu & Zhijun Zhu & Daniel G. Delafield & Michael J. Rigby & Gaoyuan Lu & Megan Braun & Luigi Puglielli & Lingjun Li, 2024. "Spatially and temporally probing distinctive glycerophospholipid alterations in Alzheimer’s disease mouse brain via high-resolution ion mobility-enabled sn-position resolved lipidomics," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    5. Zidan Wang & Donghui Zhang & Junhan Wu & Wenpeng Zhang & Yu Xia, 2024. "Illuminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    6. Mouna Chajadine & Ludivine Laurans & Tobias Radecke & Nirmala Mouttoulingam & Rida Al-Rifai & Emilie Bacquer & Clara Delaroque & Héloïse Rytter & Marius Bredon & Camille Knosp & José Vilar & Coralie F, 2024. "Harnessing intestinal tryptophan catabolism to relieve atherosclerosis in mice," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    7. Bennett W. Fox & Maximilian J. Helf & Russell N. Burkhardt & Alexander B. Artyukhin & Brian J. Curtis & Diana Fajardo Palomino & Allen F. Schroeder & Amaresh Chaturbedi & Arnaud Tauffenberger & Cheste, 2024. "Evolutionarily related host and microbial pathways regulate fat desaturation in C. elegans," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    8. Maria Osetrova & Anna Tkachev & Waltraud Mair & Patricia Guijarro Larraz & Olga Efimova & Ilia Kurochkin & Elena Stekolshchikova & Nickolay Anikanov & Juat Chin Foo & Amaury Cazenave-Gassiot & Aleksan, 2024. "Lipidome atlas of the adult human brain," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    9. Ana Carolina Dantas Machado & Stephany Flores Ramos & Julia M. Gauglitz & Anne-Marie Fassler & Daniel Petras & Alexander A. Aksenov & Un Bi Kim & Michael Lazarowicz & Abbey Barnard Giustini & Hamed Ar, 2023. "Portosystemic shunt placement reveals blood signatures for the development of hepatic encephalopathy through mass spectrometry," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    10. Kui Deng & Jin-jian Xu & Luqi Shen & Hui Zhao & Wanglong Gou & Fengzhe Xu & Yuanqing Fu & Zengliang Jiang & Menglei Shuai & Bang-yan Li & Wei Hu & Ju-Sheng Zheng & Yu-ming Chen, 2023. "Comparison of fecal and blood metabolome reveals inconsistent associations of the gut microbiota with cardiometabolic diseases," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37055-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.