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Metabolic preference assay for rapid diagnosis of bloodstream infections

Author

Listed:
  • Thomas Rydzak

    (University of Calgary)

  • Ryan A. Groves

    (University of Calgary)

  • Ruichuan Zhang

    (University of Calgary)

  • Raied Aburashed

    (University of Calgary)

  • Rajnigandha Pushpker

    (University of Calgary)

  • Maryam Mapar

    (University of Calgary)

  • Ian A. Lewis

    (University of Calgary)

Abstract

Bloodstream infections (BSIs) cause >500,000 infections and >80,000 deaths per year in North America. The length of time between the onset of symptoms and administration of appropriate antimicrobials is directly linked to mortality rates. It currently takes 2–5 days to identify BSI pathogens and measure their susceptibility to antimicrobials – a timeline that directly contributes to preventable deaths. To address this, we demonstrate a rapid metabolic preference assay (MPA) that uses the pattern of metabolic fluxes observed in ex-vivo microbial cultures to identify common pathogens and determine their antimicrobial susceptibility profiles. In a head-to-head race with a leading platform (VITEK 2, BioMérieux) used in diagnostic laboratories, MPA decreases testing timelines from 40 hours to under 20. If put into practice, this assay could reduce septic shock mortality and reduce the use of broad spectrum antibiotics.

Suggested Citation

  • Thomas Rydzak & Ryan A. Groves & Ruichuan Zhang & Raied Aburashed & Rajnigandha Pushpker & Maryam Mapar & Ian A. Lewis, 2022. "Metabolic preference assay for rapid diagnosis of bloodstream infections," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30048-6
    DOI: 10.1038/s41467-022-30048-6
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    Cited by:

    1. Kirsty Brown & Carolyn A. Thomson & Soren Wacker & Marija Drikic & Ryan Groves & Vina Fan & Ian A. Lewis & Kathy D. McCoy, 2023. "Microbiota alters the metabolome in an age- and sex- dependent manner in mice," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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