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Two-photon fluorescence imaging and specifically biosensing of norepinephrine on a 100-ms timescale

Author

Listed:
  • Leiwen Mao

    (East China Normal University)

  • Yujie Han

    (East China Normal University)

  • Qi-Wei Zhang

    (East China Normal University)

  • Yang Tian

    (East China Normal University)

Abstract

Norepinephrine (NE) is a key neurotransmitter in the central nervous system of organisms; however, specifically tracking the transient NE dynamics with high spatiotemporal resolution in living systems remains a great challenge. Herein, we develop a small molecular fluorescent probe that can precisely anchor on neuronal cytomembranes and specifically respond to NE on a 100-ms timescale. A unique dual acceleration mechanism of molecular-folding and water-bridging is disclosed, which boosts the reaction kinetics by ˃105 and ˃103 times, respectively. Benefiting from its excellent spatiotemporal resolution, the probe is applied to monitor NE dynamics at the single-neuron level, thereby, successfully snapshotting the fast fluctuation of NE levels at neuronal cytomembranes within 2 s. Moreover, two-photon fluorescence imaging of acute brain tissue slices reveals a close correlation between downregulated NE levels and Alzheimer’s disease pathology as well as antioxidant therapy.

Suggested Citation

  • Leiwen Mao & Yujie Han & Qi-Wei Zhang & Yang Tian, 2023. "Two-photon fluorescence imaging and specifically biosensing of norepinephrine on a 100-ms timescale," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36869-3
    DOI: 10.1038/s41467-023-36869-3
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    References listed on IDEAS

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    1. Shabareesh Pidathala & Aditya Kumar Mallela & Deepthi Joseph & Aravind Penmatsa, 2021. "Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Vivien Marx, 2014. "A deep look at synaptic dynamics," Nature, Nature, vol. 515(7526), pages 293-297, November.
    3. Jinxing Li & Yuxin Liu & Lei Yuan & Baibing Zhang & Estelle Spear Bishop & Kecheng Wang & Jing Tang & Yu-Qing Zheng & Wenhui Xu & Simiao Niu & Levent Beker & Thomas L. Li & Gan Chen & Modupeola Diyaol, 2022. "A tissue-like neurotransmitter sensor for the brain and gut," Nature, Nature, vol. 606(7912), pages 94-101, June.
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