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Membrane phospholipids control gating of the mechanosensitive potassium leak channel TREK1

Author

Listed:
  • Philipp A. M. Schmidpeter

    (Weill Cornell Medical College)

  • John T. Petroff

    (Washington University School of Medicine in St. Louis)

  • Leila Khajoueinejad

    (Weill Cornell Medical College)

  • Aboubacar Wague

    (Weill Cornell Medical College)

  • Cheryl Frankfater

    (Washington University School of Medicine in St. Louis)

  • Wayland W. L. Cheng

    (Washington University School of Medicine in St. Louis)

  • Crina M. Nimigean

    (Weill Cornell Medical College
    Weill Cornell Medical College
    Weill Cornell Medical College)

  • Paul M. Riegelhaupt

    (Weill Cornell Medical College)

Abstract

Tandem pore domain (K2P) potassium channels modulate resting membrane potentials and shape cellular excitability. For the mechanosensitive subfamily of K2Ps, the composition of phospholipids within the bilayer strongly influences channel activity. To examine the molecular details of K2P lipid modulation, we solved cryo-EM structures of the TREK1 K2P channel bound to either the anionic lipid phosphatidic acid (PA) or the zwitterionic lipid phosphatidylethanolamine (PE). At the extracellular face of TREK1, a PA lipid inserts its hydrocarbon tail into a pocket behind the selectivity filter, causing a structural rearrangement that recapitulates mutations and pharmacology known to activate TREK1. At the cytoplasmic face, PA and PE lipids compete to modulate the conformation of the TREK1 TM4 gating helix. Our findings demonstrate two distinct pathways by which anionic lipids enhance TREK1 activity and provide a framework for a model that integrates lipid gating with the effects of other mechanosensitive K2P modulators.

Suggested Citation

  • Philipp A. M. Schmidpeter & John T. Petroff & Leila Khajoueinejad & Aboubacar Wague & Cheryl Frankfater & Wayland W. L. Cheng & Crina M. Nimigean & Paul M. Riegelhaupt, 2023. "Membrane phospholipids control gating of the mechanosensitive potassium leak channel TREK1," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36765-w
    DOI: 10.1038/s41467-023-36765-w
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    1. Luis G. Cuello & Vishwanath Jogini & D. Marien Cortes & Albert C. Pan & Dominique G. Gagnon & Olivier Dalmas & Julio F. Cordero-Morales & Sudha Chakrapani & Benoît Roux & Eduardo Perozo, 2010. "Structural basis for the coupling between activation and inactivation gates in K+ channels," Nature, Nature, vol. 466(7303), pages 272-275, July.
    2. Ismail Soussia & Sonia El Mouridi & Dawon Kang & Alice Leclercq-Blondel & Lamyaa Khoubza & Philippe Tardy & Nora Zariohi & Marie Gendrel & Florian Lesage & Eun-Jin Kim & Delphine Bichet & Olga Andrini, 2019. "Mutation of a single residue promotes gating of vertebrate and invertebrate two-pore domain potassium channels," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    3. Stephen G. Brohawn & Ernest B. Campbell & Roderick MacKinnon, 2014. "Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel," Nature, Nature, vol. 516(7529), pages 126-130, December.
    4. Yixiao Zhang & Csaba Daday & Ruo-Xu Gu & Charles D. Cox & Boris Martinac & Bert L. Groot & Thomas Walz, 2021. "Visualization of the mechanosensitive ion channel MscS under membrane tension," Nature, Nature, vol. 590(7846), pages 509-514, February.
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