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Mutation of a single residue promotes gating of vertebrate and invertebrate two-pore domain potassium channels

Author

Listed:
  • Ismail Soussia

    (Université Claude Bernard Lyon 1)

  • Sonia El Mouridi

    (Université Claude Bernard Lyon 1)

  • Dawon Kang

    (Gyeongsang National University)

  • Alice Leclercq-Blondel

    (Université Claude Bernard Lyon 1)

  • Lamyaa Khoubza

    (Université de Nice Sophia Antipolis)

  • Philippe Tardy

    (Université Claude Bernard Lyon 1)

  • Nora Zariohi

    (Université Claude Bernard Lyon 1)

  • Marie Gendrel

    (Université Claude Bernard Lyon 1)

  • Florian Lesage

    (Université de Nice Sophia Antipolis)

  • Eun-Jin Kim

    (Gyeongsang National University)

  • Delphine Bichet

    (Université de Nice Sophia Antipolis)

  • Olga Andrini

    (Université Claude Bernard Lyon 1)

  • Thomas Boulin

    (Université Claude Bernard Lyon 1)

Abstract

Mutations that modulate the activity of ion channels are essential tools to understand the biophysical determinants that control their gating. Here, we reveal the conserved role played by a single amino acid position (TM2.6) located in the second transmembrane domain of two-pore domain potassium (K2P) channels. Mutations of TM2.6 to aspartate or asparagine increase channel activity for all vertebrate K2P channels. Using two-electrode voltage-clamp and single-channel recording techniques, we find that mutation of TM2.6 promotes channel gating via the selectivity filter gate and increases single channel open probability. Furthermore, channel gating can be progressively tuned by using different amino acid substitutions. Finally, we show that the role of TM2.6 was conserved during evolution by rationally designing gain-of-function mutations in four Caenorhabditis elegans K2P channels using CRISPR/Cas9 gene editing. This study thus describes a simple and powerful strategy to systematically manipulate the activity of an entire family of potassium channels.

Suggested Citation

  • Ismail Soussia & Sonia El Mouridi & Dawon Kang & Alice Leclercq-Blondel & Lamyaa Khoubza & Philippe Tardy & Nora Zariohi & Marie Gendrel & Florian Lesage & Eun-Jin Kim & Delphine Bichet & Olga Andrini, 2019. "Mutation of a single residue promotes gating of vertebrate and invertebrate two-pore domain potassium channels," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-08710-3
    DOI: 10.1038/s41467-019-08710-3
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    Cited by:

    1. Philipp A. M. Schmidpeter & John T. Petroff & Leila Khajoueinejad & Aboubacar Wague & Cheryl Frankfater & Wayland W. L. Cheng & Crina M. Nimigean & Paul M. Riegelhaupt, 2023. "Membrane phospholipids control gating of the mechanosensitive potassium leak channel TREK1," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Alice Peysson & Noura Zariohi & Marie Gendrel & Amandine Chambert-Loir & Noémie Frébault & Elise Cheynet & Olga Andrini & Thomas Boulin, 2024. "Wnt-Ror-Dvl signalling and the dystrophin complex organize planar-polarized membrane compartments in C. elegans muscles," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    3. Toby S. Turney & Vivian Li & Stephen G. Brohawn, 2022. "Structural Basis for pH-gating of the K+ channel TWIK1 at the selectivity filter," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

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