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Structure of the 30S ribosomal subunit

Author

Listed:
  • Brian T. Wimberly

    (MRC Laboratory of Molecular Biology)

  • Ditlev E. Brodersen

    (MRC Laboratory of Molecular Biology)

  • William M. Clemons

    (MRC Laboratory of Molecular Biology
    University of Utah School of Medicine)

  • Robert J. Morgan-Warren

    (MRC Laboratory of Molecular Biology)

  • Andrew P. Carter

    (MRC Laboratory of Molecular Biology)

  • Clemens Vonrhein

    (Global Phasing Ltd., Sheraton House)

  • Thomas Hartsch

    (Göttingen Genomics Laboratory, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen)

  • V. Ramakrishnan

    (MRC Laboratory of Molecular Biology)

Abstract

Genetic information encoded in messenger RNA is translated into protein by the ribosome, which is a large nucleoprotein complex comprising two subunits, denoted 30S and 50S in bacteria. Here we report the crystal structure of the 30S subunit from Thermus thermophilus, refined to 3 Å resolution. The final atomic model rationalizes over four decades of biochemical data on the ribosome, and provides a wealth of information about RNA and protein structure, protein–RNA interactions and ribosome assembly. It is also a structural basis for analysis of the functions of the 30S subunit, such as decoding, and for understanding the action of antibiotics. The structure will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography.

Suggested Citation

  • Brian T. Wimberly & Ditlev E. Brodersen & William M. Clemons & Robert J. Morgan-Warren & Andrew P. Carter & Clemens Vonrhein & Thomas Hartsch & V. Ramakrishnan, 2000. "Structure of the 30S ribosomal subunit," Nature, Nature, vol. 407(6802), pages 327-339, September.
    • Handle: RePEc:nat:nature:v:407:y:2000:i:6802:d:10.1038_35030006
    DOI: 10.1038/35030006
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    Cited by:

    1. Rebecca S Mathew-Fenn & Rhiju Das & Joshua A Silverman & Peter A Walker & Pehr A B Harbury, 2008. "A Molecular Ruler for Measuring Quantitative Distance Distributions," PLOS ONE, Public Library of Science, vol. 3(10), pages 1-9, October.
    2. Simon A. Fromm & Kate M. O’Connor & Michael Purdy & Pramod R. Bhatt & Gary Loughran & John F. Atkins & Ahmad Jomaa & Simone Mattei, 2023. "The translating bacterial ribosome at 1.55 Å resolution generated by cryo-EM imaging services," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    3. Mariam Jaafar & Julia Contreras & Carine Dominique & Sara Martín-Villanueva & Régine Capeyrou & Patrice Vitali & Olga Rodríguez-Galán & Carmen Velasco & Odile Humbert & Nicholas J. Watkins & Eduardo V, 2021. "Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    4. Meagan C Small & Pedro Lopes & Rodrigo B Andrade & Alexander D MacKerell Jr, 2013. "Impact of Ribosomal Modification on the Binding of the Antibiotic Telithromycin Using a Combined Grand Canonical Monte Carlo/Molecular Dynamics Simulation Approach," PLOS Computational Biology, Public Library of Science, vol. 9(6), pages 1-14, June.

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