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Single-cell transcriptomic analysis identifies murine heart molecular features at embryonic and neonatal stages

Author

Listed:
  • Wei Feng

    (University of Pittsburgh School of Medicine)

  • Abha Bais

    (University of Pittsburgh School of Medicine)

  • Haoting He

    (University of Pittsburgh School of Medicine)

  • Cassandra Rios

    (University of Pittsburgh School of Medicine)

  • Shan Jiang

    (University of Pittsburgh School of Medicine)

  • Juan Xu

    (University of Pittsburgh School of Medicine)

  • Cindy Chang

    (University of Pittsburgh School of Medicine)

  • Dennis Kostka

    (University of Pittsburgh School of Medicine
    University of Pittsburgh School of Medicine)

  • Guang Li

    (University of Pittsburgh School of Medicine)

Abstract

Heart development is a continuous process involving significant remodeling during embryogenesis and neonatal stages. To date, several groups have used single-cell sequencing to characterize the heart transcriptomes but failed to capture the progression of heart development at most stages. This has left gaps in understanding the contribution of each cell type across cardiac development. Here, we report the transcriptional profile of the murine heart from early embryogenesis to late neonatal stages. Through further analysis of this dataset, we identify several transcriptional features. We identify gene expression modules enriched at early embryonic and neonatal stages; multiple cell types in the left and right atriums are transcriptionally distinct at neonatal stages; many congenital heart defect-associated genes have cell type-specific expression; stage-unique ligand-receptor interactions are mostly between epicardial cells and other cell types at neonatal stages; and mutants of epicardium-expressed genes Wt1 and Tbx18 have different heart defects. Assessment of this dataset serves as an invaluable source of information for studies of heart development.

Suggested Citation

  • Wei Feng & Abha Bais & Haoting He & Cassandra Rios & Shan Jiang & Juan Xu & Cindy Chang & Dennis Kostka & Guang Li, 2022. "Single-cell transcriptomic analysis identifies murine heart molecular features at embryonic and neonatal stages," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35691-7
    DOI: 10.1038/s41467-022-35691-7
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    References listed on IDEAS

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    1. Fangfang Qu & Wenjia Li & Jian Xu & Ruifang Zhang & Jincan Ke & Xiaodie Ren & Xiaogao Meng & Lexin Qin & Jingna Zhang & Fangru Lu & Xin Zhou & Xi Luo & Zhen Zhang & Minhan Wang & Guangming Wu & Duanqi, 2023. "Three-dimensional molecular architecture of mouse organogenesis," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

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