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Membrane curvature governs the distribution of Piezo1 in live cells

Author

Listed:
  • Shilong Yang

    (Rutgers University)

  • Xinwen Miao

    (Nanyang Technological University)

  • Steven Arnold

    (Rutgers University)

  • Boxuan Li

    (Rutgers University)

  • Alan T. Ly

    (UC Irvine
    UC Irvine)

  • Huan Wang

    (Rutgers University)

  • Matthew Wang

    (Rutgers University)

  • Xiangfu Guo

    (Nanyang Technological University)

  • Medha M. Pathak

    (UC Irvine
    UC Irvine)

  • Wenting Zhao

    (Nanyang Technological University)

  • Charles D. Cox

    (Victor Chang Cardiac Research Institute)

  • Zheng Shi

    (Rutgers University
    Rutgers University)

Abstract

Piezo1 is a bona fide mechanosensitive ion channel ubiquitously expressed in mammalian cells. The distribution of Piezo1 within a cell is essential for various biological processes including cytokinesis, cell migration, and wound healing. However, the underlying principles that guide the subcellular distribution of Piezo1 remain largely unexplored. Here, we demonstrate that membrane curvature serves as a key regulator of the spatial distribution of Piezo1 in the plasma membrane of living cells. Piezo1 depletes from highly curved membrane protrusions such as filopodia and enriches to nanoscale membrane invaginations. Quantification of the curvature-dependent sorting of Piezo1 directly reveals the in situ nano-geometry of the Piezo1-membrane complex. Piezo1 density on filopodia increases upon activation, independent of calcium, suggesting flattening of the channel upon opening. Consequently, the expression of Piezo1 inhibits filopodia formation, an effect that diminishes with channel activation.

Suggested Citation

  • Shilong Yang & Xinwen Miao & Steven Arnold & Boxuan Li & Alan T. Ly & Huan Wang & Matthew Wang & Xiangfu Guo & Medha M. Pathak & Wenting Zhao & Charles D. Cox & Zheng Shi, 2022. "Membrane curvature governs the distribution of Piezo1 in live cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35034-6
    DOI: 10.1038/s41467-022-35034-6
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    1. Haoqing Jerry Wang & Yao Wang & Seyed Sajad Mirjavadi & Tomas Andersen & Laura Moldovan & Parham Vatankhah & Blake Russell & Jasmine Jin & Zijing Zhou & Qing Li & Charles D. Cox & Qian Peter Su & Lini, 2024. "Microscale geometrical modulation of PIEZO1 mediated mechanosensing through cytoskeletal redistribution," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Sara Baratchi & Habiba Danish & Chanly Chheang & Ying Zhou & Angela Huang & Austin Lai & Manijeh Khanmohammadi & Kylie M. Quinn & Khashayar Khoshmanesh & Karlheinz Peter, 2024. "Piezo1 expression in neutrophils regulates shear-induced NETosis," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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