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Non-equilibration of hydrostatic pressure in blebbing cells

Author

Listed:
  • Guillaume T. Charras

    (Harvard Medical School)

  • Justin C. Yarrow

    (Harvard Medical School)

  • Mike A. Horton

    (University College London)

  • L. Mahadevan

    (Harvard Medical School
    Harvard University
    Harvard University)

  • T. J. Mitchison

    (Harvard Medical School)

Abstract

Current models for protrusive motility in animal cells focus on cytoskeleton-based mechanisms, where localized protrusion is driven by local regulation of actin biochemistry1,2,3. In plants and fungi, protrusion is driven primarily by hydrostatic pressure4,5,6. For hydrostatic pressure to drive localized protrusion in animal cells7,8, it would have to be locally regulated, but current models treating cytoplasm as an incompressible viscoelastic continuum9 or viscous liquid10 require that hydrostatic pressure equilibrates essentially instantaneously over the whole cell. Here, we use cell blebs as reporters of local pressure in the cytoplasm. When we locally perfuse blebbing cells with cortex-relaxing drugs to dissipate pressure on one side, blebbing continues on the untreated side, implying non-equilibration of pressure on scales of approximately 10 µm and 10 s. We can account for localization of pressure by considering the cytoplasm as a contractile, elastic network infiltrated by cytosol. Motion of the fluid relative to the network generates spatially heterogeneous transients in the pressure field, and can be described in the framework of poroelasticity11,12.

Suggested Citation

  • Guillaume T. Charras & Justin C. Yarrow & Mike A. Horton & L. Mahadevan & T. J. Mitchison, 2005. "Non-equilibration of hydrostatic pressure in blebbing cells," Nature, Nature, vol. 435(7040), pages 365-369, May.
  • Handle: RePEc:nat:nature:v:435:y:2005:i:7040:d:10.1038_nature03550
    DOI: 10.1038/nature03550
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    Cited by:

    1. Shilong Yang & Xinwen Miao & Steven Arnold & Boxuan Li & Alan T. Ly & Huan Wang & Matthew Wang & Xiangfu Guo & Medha M. Pathak & Wenting Zhao & Charles D. Cox & Zheng Shi, 2022. "Membrane curvature governs the distribution of Piezo1 in live cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Jiu-Tao Hang & Yu Kang & Guang-Kui Xu & Huajian Gao, 2021. "A hierarchical cellular structural model to unravel the universal power-law rheological behavior of living cells," Nature Communications, Nature, vol. 12(1), pages 1-7, December.
    3. Kazuya Tsujita & Reiko Satow & Shinobu Asada & Yoshikazu Nakamura & Luis Arnes & Keisuke Sako & Yasuyuki Fujita & Kiyoko Fukami & Toshiki Itoh, 2021. "Homeostatic membrane tension constrains cancer cell dissemination by counteracting BAR protein assembly," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    4. Andreia R. Fernandes & João P. Martins & Edgar R. Gomes & César S. Mendes & Rita O. Teodoro, 2023. "Drosophila motor neuron boutons remodel through membrane blebbing coupled with muscle contraction," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

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