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Architecture of the mammalian mechanosensitive Piezo1 channel

Author

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  • Jingpeng Ge

    (Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences or Medicine, Tsinghua University
    Ministry of Education, Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University)

  • Wanqiu Li

    (Ministry of Education, Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University)

  • Qiancheng Zhao

    (Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences or Medicine, Tsinghua University
    School of Medicine, Tsinghua University)

  • Ningning Li

    (Ministry of Education, Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University)

  • Maofei Chen

    (Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences or Medicine, Tsinghua University
    Ministry of Education, Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University)

  • Peng Zhi

    (School of Medicine, Tsinghua University)

  • Ruochong Li

    (Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences or Medicine, Tsinghua University
    Ministry of Education, Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University)

  • Ning Gao

    (Ministry of Education, Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University)

  • Bailong Xiao

    (Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences or Medicine, Tsinghua University
    School of Medicine, Tsinghua University
    IDG/McGovern Institute for Brain Research, Tsinghua University)

  • Maojun Yang

    (Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences or Medicine, Tsinghua University
    Ministry of Education, Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University)

Abstract

Piezo proteins are evolutionarily conserved and functionally diverse mechanosensitive cation channels. However, the overall structural architecture and gating mechanisms of Piezo channels have remained unknown. Here we determine the cryo-electron microscopy structure of the full-length (2,547 amino acids) mouse Piezo1 (Piezo1) at a resolution of 4.8 Å. Piezo1 forms a trimeric propeller-like structure (about 900 kilodalton), with the extracellular domains resembling three distal blades and a central cap. The transmembrane region has 14 apparently resolved segments per subunit. These segments form three peripheral wings and a central pore module that encloses a potential ion-conducting pore. The rather flexible extracellular blade domains are connected to the central intracellular domain by three long beam-like structures. This trimeric architecture suggests that Piezo1 may use its peripheral regions as force sensors to gate the central ion-conducting pore.

Suggested Citation

  • Jingpeng Ge & Wanqiu Li & Qiancheng Zhao & Ningning Li & Maofei Chen & Peng Zhi & Ruochong Li & Ning Gao & Bailong Xiao & Maojun Yang, 2015. "Architecture of the mammalian mechanosensitive Piezo1 channel," Nature, Nature, vol. 527(7576), pages 64-69, November.
    • Handle: RePEc:nat:nature:v:527:y:2015:i:7576:d:10.1038_nature15247
    DOI: 10.1038/nature15247
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    Cited by:

    1. Shilong Yang & Xinwen Miao & Steven Arnold & Boxuan Li & Alan T. Ly & Huan Wang & Matthew Wang & Xiangfu Guo & Medha M. Pathak & Wenting Zhao & Charles D. Cox & Zheng Shi, 2022. "Membrane curvature governs the distribution of Piezo1 in live cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Amandeep Kaur & Madhu & Alok Sharma & Kashmir Singh & Santosh Kumar Upadhyay, 2023. "Exploration of Piezo Channels in Bread Wheat ( Triticum aestivum L.)," Agriculture, MDPI, vol. 13(4), pages 1-16, March.
    3. Jeong Han Lee & Maria C. Perez-Flores & Seojin Park & Hyo Jeong Kim & Yingying Chen & Mincheol Kang & Jennifer Kersigo & Jinsil Choi & Phung N. Thai & Ryan L. Woltz & Dolores Columba Perez-Flores & Gu, 2024. "The Piezo channel is a mechano-sensitive complex component in the mammalian inner ear hair cell," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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