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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitro

Author

Listed:
  • Robin Caire

    (Université Claude Bernard Lyon 1
    Université Jean Monnet St-Etienne)

  • Estelle Audoux

    (Université Claude Bernard Lyon 1
    Université Jean Monnet St-Etienne)

  • Mireille Thomas

    (Université Jean Monnet St-Etienne)

  • Elisa Dalix

    (Université Jean Monnet St-Etienne)

  • Aurélien Peyron

    (Université Claude Bernard Lyon 1
    Université Jean Monnet St-Etienne)

  • Killian Rodriguez

    (Université Claude Bernard Lyon 1
    Université Jean Monnet St-Etienne)

  • Nicola Pordone

    (Université Claude Bernard Lyon 1
    Université Jean Monnet St-Etienne)

  • Johann Guillemot

    (Université Claude Bernard Lyon 1
    Université Jean Monnet St-Etienne)

  • Yann Dickerscheit

    (Université Claude Bernard Lyon 1
    Université Jean Monnet St-Etienne)

  • Hubert Marotte

    (Université Jean Monnet St-Etienne)

  • François Vandenesch

    (Université Claude Bernard Lyon 1
    Hospices Civiles de Lyon)

  • Frédéric Laurent

    (Université Claude Bernard Lyon 1
    Hospices Civiles de Lyon)

  • Jérôme Josse

    (Université Claude Bernard Lyon 1)

  • Paul O. Verhoeven

    (Université Claude Bernard Lyon 1
    Université Jean Monnet St-Etienne
    University Hospital of St-Etienne)

Abstract

Transcriptional cofactors YAP/TAZ have recently been found to support autophagy and inflammation, which are part of cell-autonomous immunity and are critical in antibacterial defense. Here, we studied the role of YAP against Staphylococcus aureus using CRISPR/Cas9-mutated HEK293 cells and a primary cell-based organoid model. We found that S. aureus infection increases YAP transcriptional activity, which is required to reduce intracellular S. aureus replication. A 770-gene targeted transcriptomic analysis revealed that YAP upregulates genes involved in autophagy/lysosome and inflammation pathways in both infected and uninfected conditions. The YAP-TEAD transcriptional activity promotes autophagic flux and lysosomal acidification, which are then important for defense against intracellular S. aureus. Furthermore, the staphylococcal toxin C3 exoenzyme EDIN-B was found effective in preventing YAP-mediated cell-autonomous immune response. This study provides key insights on the anti-S. aureus activity of YAP, which could be conserved for defense against other intracellular bacteria.

Suggested Citation

  • Robin Caire & Estelle Audoux & Mireille Thomas & Elisa Dalix & Aurélien Peyron & Killian Rodriguez & Nicola Pordone & Johann Guillemot & Yann Dickerscheit & Hubert Marotte & François Vandenesch & Fréd, 2022. "YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitro," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34432-0
    DOI: 10.1038/s41467-022-34432-0
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