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Circuit-guided population acclimation of a synthetic microbial consortium for improved biochemical production

Author

Listed:
  • Chae Won Kang

    (Pohang University of Science and Technology)

  • Hyun Gyu Lim

    (Pohang University of Science and Technology)

  • Jaehyuk Won

    (Chung-Ang University
    Chung-Ang University)

  • Sanghak Cha

    (Pohang University of Science and Technology)

  • Giyoung Shin

    (Pohang University of Science and Technology)

  • Jae-Seong Yang

    (Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra)

  • Jaeyoung Sung

    (Chung-Ang University
    Chung-Ang University)

  • Gyoo Yeol Jung

    (Pohang University of Science and Technology
    Pohang University of Science and Technology)

Abstract

Microbial consortia have been considered potential platforms for bioprocessing applications. However, the complexity in process control owing to the use of multiple strains necessitates the use of an efficient population control strategy. Herein, we report circuit-guided synthetic acclimation as a strategy to improve biochemical production by a microbial consortium. We designed a consortium comprising alginate-utilizing Vibrio sp. dhg and 3-hydroxypropionic acid (3-HP)-producing Escherichia coli strains for the direct conversion of alginate to 3-HP. We introduced a genetic circuit, named “Population guider”, in the E. coli strain, which degrades ampicillin only when 3-HP is produced. In the presence of ampicillin as a selection pressure, the consortium was successfully acclimated for increased 3-HP production by 4.3-fold compared to that by a simple co-culturing consortium during a 48-h fermentation. We believe this concept is a useful strategy for the development of robust consortium-based bioprocesses.

Suggested Citation

  • Chae Won Kang & Hyun Gyu Lim & Jaehyuk Won & Sanghak Cha & Giyoung Shin & Jae-Seong Yang & Jaeyoung Sung & Gyoo Yeol Jung, 2022. "Circuit-guided population acclimation of a synthetic microbial consortium for improved biochemical production," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34190-z
    DOI: 10.1038/s41467-022-34190-z
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    References listed on IDEAS

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    1. Lingchong You & Robert Sidney Cox & Ron Weiss & Frances H. Arnold, 2004. "Programmed population control by cell–cell communication and regulated killing," Nature, Nature, vol. 428(6985), pages 868-871, April.
    2. Kristina Stephens & Maria Pozo & Chen-Yu Tsao & Pricila Hauk & William E. Bentley, 2019. "Bacterial co-culture with cell signaling translator and growth controller modules for autonomously regulated culture composition," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    3. Hyun Gyu Lim & Dong Hun Kwak & Sungwoo Park & Sunghwa Woo & Jae-Seong Yang & Chae Won Kang & Beomhee Kim & Myung Hyun Noh & Sang Woo Seo & Gyoo Yeol Jung, 2019. "Vibrio sp. dhg as a platform for the biorefinery of brown macroalgae," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    4. Nicolas Kylilis & Zoltan A. Tuza & Guy-Bart Stan & Karen M. Polizzi, 2018. "Tools for engineering coordinated system behaviour in synthetic microbial consortia," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
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    Cited by:

    1. Gita Naseri, 2023. "A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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