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Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states

Author

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  • Yingying Guo

    (Westlake University
    Westlake Institute for Advanced Study
    Southern University of Science and Technology)

  • Yuanyuan Zhang

    (Westlake University
    Westlake Institute for Advanced Study)

  • Renhong Yan

    (Westlake University
    Westlake Institute for Advanced Study
    Southern University of Science and Technology)

  • Bangdong Huang

    (Westlake University
    Westlake Institute for Advanced Study)

  • Fangfei Ye

    (Westlake University
    Westlake Institute for Advanced Study)

  • Liushu Wu

    (Westlake University
    Westlake Institute for Advanced Study)

  • Ximin Chi

    (Westlake University
    Westlake Institute for Advanced Study)

  • Yi shi

    (Westlake University
    Westlake Institute for Advanced Study)

  • Qiang Zhou

    (Westlake University
    Westlake Institute for Advanced Study)

Abstract

Sodium-Potassium Pump (Na+/K+-ATPase, NKA) is an ion pump that generates an electrochemical gradient of sodium and potassium ions across the plasma membrane by hydrolyzing ATP. During each Post-Albers cycle, NKA exchanges three cytoplasmic sodium ions for two extracellular potassium ions through alternating changes between the E1 and E2 states. Hitherto, several steps remained unknown during the complete working cycle of NKA. Here, we report cryo-electron microscopy (cryo-EM) structures of recombinant human NKA (hNKA) in three distinct states at 2.7–3.2 Å resolution, representing the E1·3Na and E1·3Na·ATP states with cytosolic gates open and the basic E2·[2K] state, respectively. This work provides the insights into the cytoplasmic Na+ entrance pathway and the mechanism of cytoplasmic gate closure coupled with ATP hydrolysis, filling crucial gaps in the structural elucidation of the Post-Albers cycle of NKA.

Suggested Citation

  • Yingying Guo & Yuanyuan Zhang & Renhong Yan & Bangdong Huang & Fangfei Ye & Liushu Wu & Ximin Chi & Yi shi & Qiang Zhou, 2022. "Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31602-y
    DOI: 10.1038/s41467-022-31602-y
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    References listed on IDEAS

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