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Brain-wide projection reconstruction of single functionally defined neurons

Author

Listed:
  • Meng Wang

    (Third Military Medical University
    Third Military Medical University
    Chongqing University)

  • Ke Liu

    (Third Military Medical University
    Third Military Medical University)

  • Junxia Pan

    (Third Military Medical University
    Third Military Medical University)

  • Jialin Li

    (Chongqing University)

  • Pei Sun

    (Third Military Medical University
    Third Military Medical University)

  • Yongsheng Zhang

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Longhui Li

    (Chongqing University
    Guangxi University)

  • Wenyan Guo

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Qianqian Xin

    (Third Military Medical University
    Third Military Medical University)

  • Zhikai Zhao

    (Third Military Medical University
    Third Military Medical University)

  • Yurong Liu

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Zhenqiao Zhou

    (Chinese Academy of Sciences)

  • Jing Lyu

    (Chinese Academy of Sciences)

  • Ting Zheng

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Yunyun Han

    (School of Basic Medicine and Tongji Medical College, Huazhong University of Science & Technology)

  • Chunqing Zhang

    (Third Military Medical University
    Third Military Medical University)

  • Xiang Liao

    (Chongqing University)

  • Shaoqun Zeng

    (Huazhong University of Science and Technology
    Huazhong University of Science and Technology)

  • Hongbo Jia

    (Guangxi University
    Chinese Academy of Sciences
    Technical University Munich
    Leibniz Institute for Neurobiology)

  • Xiaowei Chen

    (Third Military Medical University
    Third Military Medical University
    Chinese Academy of Sciences
    Guangyang Bay Laboratory)

Abstract

Reconstructing axonal projections of single neurons at the whole-brain level is currently a converging goal of the neuroscience community that is fundamental for understanding the logic of information flow in the brain. Thousands of single neurons from different brain regions have recently been morphologically reconstructed, but the corresponding physiological functional features of these reconstructed neurons are unclear. By combining two-photon Ca2+ imaging with targeted single-cell plasmid electroporation, we reconstruct the brain-wide morphologies of single neurons that are defined by a sound-evoked response map in the auditory cortices (AUDs) of awake mice. Long-range interhemispheric projections can be reliably labelled via co-injection with an adeno-associated virus, which enables enhanced expression of indicator protein in the targeted neurons. Here we show that this method avoids the randomness and ambiguity of conventional methods of neuronal morphological reconstruction, offering an avenue for developing a precise one-to-one map of neuronal projection patterns and physiological functional features.

Suggested Citation

  • Meng Wang & Ke Liu & Junxia Pan & Jialin Li & Pei Sun & Yongsheng Zhang & Longhui Li & Wenyan Guo & Qianqian Xin & Zhikai Zhao & Yurong Liu & Zhenqiao Zhou & Jing Lyu & Ting Zheng & Yunyun Han & Chunq, 2022. "Brain-wide projection reconstruction of single functionally defined neurons," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29229-0
    DOI: 10.1038/s41467-022-29229-0
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