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Structures of a deltacoronavirus spike protein bound to porcine and human receptors

Author

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  • Weiwei Ji

    (Nanjing Agricultural University)

  • Qi Peng

    (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture
    Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology
    Yangzhou University)

  • Xueqiong Fang

    (Nanjing Agricultural University)

  • Zehou Li

    (Nanjing Agricultural University)

  • Yaxin Li

    (Nanjing Agricultural University)

  • Cunfa Xu

    (Central Laboratory of Jiangsu Academy of Agricultural Sciences)

  • Shuqing Zhao

    (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture
    Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology
    Yangzhou University)

  • Jizong Li

    (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture
    Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology
    Yangzhou University)

  • Rong Chen

    (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture)

  • Guoxiang Mo

    (Nanjing Agricultural University)

  • Zhanyong Wei

    (College of Veterinary Medicine, Henan Agricultural University)

  • Ying Xu

    (Nanjing Agricultural University)

  • Bin Li

    (Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture
    Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology
    Yangzhou University)

  • Shuijun Zhang

    (Nanjing Agricultural University)

Abstract

Porcine deltacoronavirus (PDCoV) can experimentally infect a variety of animals. Human infection by PDCoV has also been reported. Consistently, PDCoV can use aminopeptidase N (APN) from different host species as receptors to enter cells. To understand this broad receptor usage and interspecies transmission of PDCoV, we determined the crystal structures of the receptor binding domain (RBD) of PDCoV spike protein bound to human APN (hAPN) and porcine APN (pAPN), respectively. The structures of the two complexes exhibit high similarity. PDCoV RBD binds to common regions on hAPN and pAPN, which are different from the sites engaged by two alphacoronaviruses: HCoV-229E and porcine respiratory coronavirus (PRCoV). Based on structure guided mutagenesis, we identified conserved residues on hAPN and pAPN that are essential for PDCoV binding and infection. We report the detailed mechanism for how a deltacoronavirus recognizes homologous receptors and provide insights into the cross-species transmission of PDCoV.

Suggested Citation

  • Weiwei Ji & Qi Peng & Xueqiong Fang & Zehou Li & Yaxin Li & Cunfa Xu & Shuqing Zhao & Jizong Li & Rong Chen & Guoxiang Mo & Zhanyong Wei & Ying Xu & Bin Li & Shuijun Zhang, 2022. "Structures of a deltacoronavirus spike protein bound to porcine and human receptors," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29062-5
    DOI: 10.1038/s41467-022-29062-5
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    1. Wenjuan Du & Oliver Debski-Antoniak & Dubravka Drabek & Rien Haperen & Melissa Dortmondt & Joline Lee & Ieva Drulyte & Frank J. M. Kuppeveld & Frank Grosveld & Daniel L. Hurdiss & Berend-Jan Bosch, 2024. "Neutralizing antibodies reveal cryptic vulnerabilities and interdomain crosstalk in the porcine deltacoronavirus spike protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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