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Two zinc finger proteins with functions in m6A writing interact with HAKAI

Author

Listed:
  • Mi Zhang

    (University of Nottingham)

  • Zsuzsanna Bodi

    (University of Nottingham)

  • Katarzyna Mackinnon

    (University of Dundee)

  • Silin Zhong

    (University of Nottingham
    The Chinese University of Hong Kong)

  • Nathan Archer

    (University of Nottingham)

  • Nigel P. Mongan

    (University of Nottingham
    Weill Cornell Medicine)

  • Gordon G. Simpson

    (University of Dundee)

  • Rupert G. Fray

    (University of Nottingham)

Abstract

The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Here, using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, are discovered as components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA (mRNA adenosine methylase A). Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation and decreased lateral root formation. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ2 is likely the plant equivalent of ZC3H13 (Flacc) of the metazoan m6A-METTL Associated Complex.

Suggested Citation

  • Mi Zhang & Zsuzsanna Bodi & Katarzyna Mackinnon & Silin Zhong & Nathan Archer & Nigel P. Mongan & Gordon G. Simpson & Rupert G. Fray, 2022. "Two zinc finger proteins with functions in m6A writing interact with HAKAI," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28753-3
    DOI: 10.1038/s41467-022-28753-3
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    References listed on IDEAS

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    1. Deepak P. Patil & Chun-Kan Chen & Brian F. Pickering & Amy Chow & Constanza Jackson & Mitchell Guttman & Samie R. Jaffrey, 2016. "m6A RNA methylation promotes XIST-mediated transcriptional repression," Nature, Nature, vol. 537(7620), pages 369-373, September.
    2. Irmgard U. Haussmann & Zsuzsanna Bodi & Eugenio Sanchez-Moran & Nigel P. Mongan & Nathan Archer & Rupert G. Fray & Matthias Soller, 2016. "m6A potentiates Sxl alternative pre-mRNA splicing for robust Drosophila sex determination," Nature, Nature, vol. 540(7632), pages 301-304, December.
    3. Rahul Bhosale & Jitender Giri & Bipin K. Pandey & Ricardo F. H. Giehl & Anja Hartmann & Richard Traini & Jekaterina Truskina & Nicola Leftley & Meredith Hanlon & Kamal Swarup & Afaf Rashed & Ute Voß &, 2018. "A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    4. Tina Lence & Junaid Akhtar & Marc Bayer & Katharina Schmid & Laura Spindler & Cheuk Hei Ho & Nastasja Kreim & Miguel A. Andrade-Navarro & Burkhard Poeck & Mark Helm & Jean-Yves Roignant, 2016. "m6A modulates neuronal functions and sex determination in Drosophila," Nature, Nature, vol. 540(7632), pages 242-247, December.
    5. Rahul Bhosale & Jitender Giri & Bipin K. Pandey & Ricardo F. H. Giehl & Anja Hartmann & Richard Traini & Jekaterina Truskina & Nicola Leftley & Meredith Hanlon & Kamal Swarup & Afaf Rashed & Ute Voß &, 2018. "Author Correction: A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate," Nature Communications, Nature, vol. 9(1), pages 1-1, December.
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