IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-28753-3.html
   My bibliography  Save this article

Two zinc finger proteins with functions in m6A writing interact with HAKAI

Author

Listed:
  • Mi Zhang

    (University of Nottingham)

  • Zsuzsanna Bodi

    (University of Nottingham)

  • Katarzyna Mackinnon

    (University of Dundee)

  • Silin Zhong

    (University of Nottingham
    The Chinese University of Hong Kong)

  • Nathan Archer

    (University of Nottingham)

  • Nigel P. Mongan

    (University of Nottingham
    Weill Cornell Medicine)

  • Gordon G. Simpson

    (University of Dundee)

  • Rupert G. Fray

    (University of Nottingham)

Abstract

The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Here, using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, are discovered as components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA (mRNA adenosine methylase A). Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation and decreased lateral root formation. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ2 is likely the plant equivalent of ZC3H13 (Flacc) of the metazoan m6A-METTL Associated Complex.

Suggested Citation

  • Mi Zhang & Zsuzsanna Bodi & Katarzyna Mackinnon & Silin Zhong & Nathan Archer & Nigel P. Mongan & Gordon G. Simpson & Rupert G. Fray, 2022. "Two zinc finger proteins with functions in m6A writing interact with HAKAI," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28753-3
    DOI: 10.1038/s41467-022-28753-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-28753-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-28753-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Tina Lence & Junaid Akhtar & Marc Bayer & Katharina Schmid & Laura Spindler & Cheuk Hei Ho & Nastasja Kreim & Miguel A. Andrade-Navarro & Burkhard Poeck & Mark Helm & Jean-Yves Roignant, 2016. "m6A modulates neuronal functions and sex determination in Drosophila," Nature, Nature, vol. 540(7632), pages 242-247, December.
    2. Rahul Bhosale & Jitender Giri & Bipin K. Pandey & Ricardo F. H. Giehl & Anja Hartmann & Richard Traini & Jekaterina Truskina & Nicola Leftley & Meredith Hanlon & Kamal Swarup & Afaf Rashed & Ute Voß &, 2018. "Author Correction: A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate," Nature Communications, Nature, vol. 9(1), pages 1-1, December.
    3. Deepak P. Patil & Chun-Kan Chen & Brian F. Pickering & Amy Chow & Constanza Jackson & Mitchell Guttman & Samie R. Jaffrey, 2016. "m6A RNA methylation promotes XIST-mediated transcriptional repression," Nature, Nature, vol. 537(7620), pages 369-373, September.
    4. Irmgard U. Haussmann & Zsuzsanna Bodi & Eugenio Sanchez-Moran & Nigel P. Mongan & Nathan Archer & Rupert G. Fray & Matthias Soller, 2016. "m6A potentiates Sxl alternative pre-mRNA splicing for robust Drosophila sex determination," Nature, Nature, vol. 540(7632), pages 301-304, December.
    5. Rahul Bhosale & Jitender Giri & Bipin K. Pandey & Ricardo F. H. Giehl & Anja Hartmann & Richard Traini & Jekaterina Truskina & Nicola Leftley & Meredith Hanlon & Kamal Swarup & Afaf Rashed & Ute Voß &, 2018. "A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhiyuan Luo & Jiacheng Zhang & Jingyi Fei & Shengdong Ke, 2022. "Deep learning modeling m6A deposition reveals the importance of downstream cis-element sequences," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Javier Martínez Pacheco & Philippe Ranocha & Luciana Kasulin & Corina M. Fusari & Lucas Servi & Ariel. A. Aptekmann & Victoria Berdion Gabarain & Juan Manuel Peralta & Cecilia Borassi & Eliana Marzol , 2022. "Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Rodolfo A. Maniero & Cristiana Picco & Anja Hartmann & Felipe Engelberger & Antonella Gradogna & Joachim Scholz-Starke & Michael Melzer & Georg Künze & Armando Carpaneto & Nicolaus Wirén & Ricardo F. , 2024. "Ferric reduction by a CYBDOM protein counteracts increased iron availability in root meristems induced by phosphorus deficiency," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    4. Lisa-Marie Appel & Vedran Franke & Johannes Benedum & Irina Grishkovskaya & Xué Strobl & Anton Polyansky & Gregor Ammann & Sebastian Platzer & Andrea Neudolt & Anna Wunder & Lena Walch & Stefanie Kais, 2023. "The SPOC domain is a phosphoserine binding module that bridges transcription machinery with co- and post-transcriptional regulators," Nature Communications, Nature, vol. 14(1), pages 1-22, December.
    5. P Acera Mateos & A J Sethi & A Ravindran & A Srivastava & K Woodward & S Mahmud & M Kanchi & M Guarnacci & J Xu & Z W S Yuen & Y Zhou & A Sneddon & W Hamilton & J Gao & L M Starrs & R Hayashi & V Wick, 2024. "Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence of RNA modifications," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    6. Jorge Mata-Garrido & Yao Xiang & Yunhua Chang-Marchand & Caroline Reisacher & Elisabeth Ageron & Ida Chiara Guerrera & Iñigo Casafont & Aurelia Bruneau & Claire Cherbuy & Xavier Treton & Anne Dumay & , 2022. "The Heterochromatin protein 1 is a regulator in RNA splicing precision deficient in ulcerative colitis," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    7. Hyun Jung Hwang & Tae Lim Park & Hyeong-In Kim & Yeonkyoung Park & Geunhee Kim & Chiyeol Song & Won-Ki Cho & Yoon Ki Kim, 2023. "YTHDF2 facilitates aggresome formation via UPF1 in an m6A-independent manner," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    8. Lisa-Marie Appel & Vedran Franke & Melania Bruno & Irina Grishkovskaya & Aiste Kasiliauskaite & Tanja Kaufmann & Ursula E. Schoeberl & Martin G. Puchinger & Sebastian Kostrhon & Carmen Ebenwaldner & M, 2021. "PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC," Nature Communications, Nature, vol. 12(1), pages 1-24, December.
    9. Huaxia Shi & Ying Xu & Na Tian & Ming Yang & Fu-Sen Liang, 2022. "Inducible and reversible RNA N6-methyladenosine editing," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    10. Irmgard U. Haussmann & Yanying Wu & Mohanakarthik P. Nallasivan & Nathan Archer & Zsuzsanna Bodi & Daniel Hebenstreit & Scott Waddell & Rupert Fray & Matthias Soller, 2022. "CMTr cap-adjacent 2′-O-ribose mRNA methyltransferases are required for reward learning and mRNA localization to synapses," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    11. Guoqiang Zhang & Yongru Xu & Xiaona Wang & Yuanxiang Zhu & Liangliang Wang & Wenxin Zhang & Yiru Wang & Yajie Gao & Xuna Wu & Ying Cheng & Qinmiao Sun & Dahua Chen, 2022. "Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28753-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.