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Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo

Author

Listed:
  • Yuki Bando

    (Columbia University
    Hamamatsu University School of Medicine)

  • Michael Wenzel

    (Columbia University
    University of Bonn)

  • Rafael Yuste

    (Columbia University)

Abstract

To better understand the input-output computations of neuronal populations, we developed ArcLight-ST, a genetically-encoded voltage indicator, to specifically measure subthreshold membrane potentials. We combined two-photon imaging of voltage and calcium, and successfully discriminated subthreshold inputs and spikes with cellular resolution in vivo. We demonstrate the utility of the method by mapping epileptic seizures progression through cortical circuits, revealing divergent sub- and suprathreshold dynamics within compartmentalized epileptic micronetworks. Two-photon, two-color imaging of calcium and voltage enables mapping of inputs and outputs in neuronal populations in living animals.

Suggested Citation

  • Yuki Bando & Michael Wenzel & Rafael Yuste, 2021. "Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27444-9
    DOI: 10.1038/s41467-021-27444-9
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    References listed on IDEAS

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    4. Catherine A. Schevon & Shennan A. Weiss & Guy McKhann & Robert R. Goodman & Rafael Yuste & Ronald G. Emerson & Andrew J. Trevelyan, 2012. "Evidence of an inhibitory restraint of seizure activity in humans," Nature Communications, Nature, vol. 3(1), pages 1-11, January.
    5. Yoav Adam & Jeong J. Kim & Shan Lou & Yongxin Zhao & Michael E. Xie & Daan Brinks & Hao Wu & Mohammed A. Mostajo-Radji & Simon Kheifets & Vicente Parot & Selmaan Chettih & Katherine J. Williams & Benj, 2019. "Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics," Nature, Nature, vol. 569(7756), pages 413-417, May.
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    Cited by:

    1. Ruth R. Sims & Imane Bendifallah & Christiane Grimm & Aysha S. Mohamed Lafirdeen & Soledad Domínguez & Chung Yuen Chan & Xiaoyu Lu & Benoît C. Forget & François St-Pierre & Eirini Papagiakoumou & Vale, 2024. "Scanless two-photon voltage imaging," Nature Communications, Nature, vol. 15(1), pages 1-22, December.

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