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Simultaneous dendritic voltage and calcium imaging and somatic recording from Purkinje neurons in awake mice

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  • Christopher J. Roome

    (Okinawa Institute of Science and Technology Graduate University (OIST))

  • Bernd Kuhn

    (Okinawa Institute of Science and Technology Graduate University (OIST))

Abstract

Spatiotemporal maps of dendritic signalling and their relationship with somatic output is fundamental to neuronal information processing, yet remain unexplored in awake animals. Here, we combine simultaneous sub-millisecond voltage and calcium two-photon imaging from distal spiny dendrites, with somatic electrical recording from spontaneously active cerebellar Purkinje neurons (PN) in awake mice. We detect discrete 1−2 ms suprathreshold voltage spikelets in the distal spiny dendrites during dendritic complex spikes. Spikelets and their calcium correlates are highly heterogeneous in number, timing and spatial distribution within and between complex spikes. Back-propagating simple spikes are highly attenuated. Highly variable 5–10 ms voltage hotspots are localized to fine dendritic processes and are reduced in size and frequency by lidocaine and CNQX. Hotspots correlated with somatic output but also, at high frequency, trigger purely dendritic calcium spikes. Summarizing, spatiotemporal signalling in PNs is far more complex, dynamic, and fine scaled than anticipated, even in resting animals.

Suggested Citation

  • Christopher J. Roome & Bernd Kuhn, 2018. "Simultaneous dendritic voltage and calcium imaging and somatic recording from Purkinje neurons in awake mice," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05900-3
    DOI: 10.1038/s41467-018-05900-3
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    Cited by:

    1. Yuki Bando & Michael Wenzel & Rafael Yuste, 2021. "Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Ting-Feng Lin & Silas E. Busch & Christian Hansel, 2024. "Intrinsic and synaptic determinants of receptive field plasticity in Purkinje cells of the mouse cerebellum," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    3. Ruth R. Sims & Imane Bendifallah & Christiane Grimm & Aysha S. Mohamed Lafirdeen & Soledad Domínguez & Chung Yuen Chan & Xiaoyu Lu & Benoît C. Forget & François St-Pierre & Eirini Papagiakoumou & Vale, 2024. "Scanless two-photon voltage imaging," Nature Communications, Nature, vol. 15(1), pages 1-22, December.

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