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All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals

Author

Listed:
  • Amelie C. F. Bergs

    (Goethe University
    Goethe University)

  • Jana F. Liewald

    (Goethe University
    Goethe University)

  • Silvia Rodriguez-Rozada

    (University Medical Center Hamburg-Eppendorf)

  • Qiang Liu

    (The Rockefeller University
    City University of Hong Kong)

  • Christin Wirt

    (Goethe University
    Goethe University)

  • Artur Bessel

    (Independent Researcher)

  • Nadja Zeitzschel

    (Goethe University
    Goethe University)

  • Hilal Durmaz

    (Goethe University
    Goethe University)

  • Adrianna Nozownik

    (University Medical Center Hamburg-Eppendorf)

  • Holger Dill

    (Goethe University
    Goethe University)

  • Maëlle Jospin

    (Université Claude Bernard Lyon 1, Institut NeuroMyoGène)

  • Johannes Vierock

    (Humboldt University)

  • Cornelia I. Bargmann

    (The Rockefeller University
    Chan Zuckerberg Initiative)

  • Peter Hegemann

    (Humboldt University)

  • J. Simon Wiegert

    (University Medical Center Hamburg-Eppendorf
    University of Heidelberg)

  • Alexander Gottschalk

    (Goethe University
    Goethe University)

Abstract

Excitable cells can be stimulated or inhibited by optogenetics. Since optogenetic actuation regimes are often static, neurons and circuits can quickly adapt, allowing perturbation, but not true control. Hence, we established an optogenetic voltage-clamp (OVC). The voltage-indicator QuasAr2 provides information for fast, closed-loop optical feedback to the bidirectional optogenetic actuator BiPOLES. Voltage-dependent fluorescence is held within tight margins, thus clamping the cell to distinct potentials. We established the OVC in muscles and neurons of Caenorhabditis elegans, and transferred it to rat hippocampal neurons in slice culture. Fluorescence signals were calibrated to electrically measured potentials, and wavelengths to currents, enabling to determine optical I/V-relationships. The OVC reports on homeostatically altered cellular physiology in mutants and on Ca2+-channel properties, and can dynamically clamp spiking in C. elegans. Combining non-invasive imaging with control capabilities of electrophysiology, the OVC facilitates high-throughput, contact-less electrophysiology in individual cells and paves the way for true optogenetic control in behaving animals.

Suggested Citation

  • Amelie C. F. Bergs & Jana F. Liewald & Silvia Rodriguez-Rozada & Qiang Liu & Christin Wirt & Artur Bessel & Nadja Zeitzschel & Hilal Durmaz & Adrianna Nozownik & Holger Dill & Maëlle Jospin & Johannes, 2023. "All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37622-6
    DOI: 10.1038/s41467-023-37622-6
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    References listed on IDEAS

    as
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