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Histone variant H2A.Z regulates zygotic genome activation

Author

Listed:
  • Dafne Ibarra-Morales

    (Max Planck Institute of Immunobiology and Epigenetics
    University of Freiburg, Faculty of Biology)

  • Michael Rauer

    (Max Planck Institute of Immunobiology and Epigenetics)

  • Piergiuseppe Quarato

    (Institut Pasteur, Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, UMR3738, CNRS)

  • Leily Rabbani

    (Max Planck Institute of Immunobiology and Epigenetics)

  • Fides Zenk

    (Max Planck Institute of Immunobiology and Epigenetics
    University of Freiburg, Faculty of Biology
    Department of Biosystems Science and Engineering ETH (D-BSSE ETH Zürich))

  • Mariana Schulte-Sasse

    (Max Planck Institute of Immunobiology and Epigenetics
    University of Freiburg, Faculty of Biology)

  • Francesco Cardamone

    (Max Planck Institute of Immunobiology and Epigenetics
    University of Freiburg, Faculty of Biology)

  • Alejandro Gomez-Auli

    (Max Planck Institute of Immunobiology and Epigenetics)

  • Germano Cecere

    (Institut Pasteur, Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, UMR3738, CNRS)

  • Nicola Iovino

    (Max Planck Institute of Immunobiology and Epigenetics)

Abstract

During embryogenesis, the genome shifts from transcriptionally quiescent to extensively active in a process known as Zygotic Genome Activation (ZGA). In Drosophila, the pioneer factor Zelda is known to be essential for the progression of development; still, it regulates the activation of only a small subset of genes at ZGA. However, thousands of genes do not require Zelda, suggesting that other mechanisms exist. By conducting GRO-seq, HiC and ChIP-seq in Drosophila embryos, we demonstrate that up to 65% of zygotically activated genes are enriched for the histone variant H2A.Z. H2A.Z enrichment precedes ZGA and RNA Polymerase II loading onto chromatin. In vivo knockdown of maternally contributed Domino, a histone chaperone and ATPase, reduces H2A.Z deposition at transcription start sites, causes global downregulation of housekeeping genes at ZGA, and compromises the establishment of the 3D chromatin structure. We infer that H2A.Z is essential for the de novo establishment of transcriptional programs during ZGA via chromatin reorganization.

Suggested Citation

  • Dafne Ibarra-Morales & Michael Rauer & Piergiuseppe Quarato & Leily Rabbani & Fides Zenk & Mariana Schulte-Sasse & Francesco Cardamone & Alejandro Gomez-Auli & Germano Cecere & Nicola Iovino, 2021. "Histone variant H2A.Z regulates zygotic genome activation," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27125-7
    DOI: 10.1038/s41467-021-27125-7
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    References listed on IDEAS

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    1. Xiaoqing Nie & Qianhua Xu & Chengpeng Xu & Fengling Chen & Qizhi Wang & Dandan Qin & Rui Wang & Zheng Gao & Xukun Lu & Xinai Yang & Yu Wu & Chen Gu & Wei Xie & Lei Li, 2023. "Maternal TDP-43 interacts with RNA Pol II and regulates zygotic genome activation," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Shuxiang Li & Tiejun Wei & Anna R. Panchenko, 2023. "Histone variant H2A.Z modulates nucleosome dynamics to promote DNA accessibility," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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