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Specialized transendothelial dendritic cells mediate thymic T-cell selection against blood-borne macromolecules

Author

Listed:
  • Elisabeth H. Vollmann

    (Harvard Medical School
    Merck Research Laboratories)

  • Kristin Rattay

    (Harvard Medical School
    University of Marburg)

  • Olga Barreiro

    (Harvard Medical School)

  • Aude Thiriot

    (Harvard Medical School)

  • Rebecca A. Fuhlbrigge

    (Harvard Medical School)

  • Vladimir Vrbanac

    (Massachusetts General Hospital
    Humanized Immune System Mouse Program (HISMP))

  • Ki-Wook Kim

    (Weizmann Institute of Science
    University of Illinois College of Medicine)

  • Steffen Jung

    (Weizmann Institute of Science)

  • Andrew M. Tager

    (Massachusetts General Hospital)

  • Ulrich H. von Andrian

    (Harvard Medical School
    MIT and Harvard)

Abstract

T cells undergo rigorous selection in the thymus to ensure self-tolerance and prevent autoimmunity, with this process requiring innocuous self-antigens (Ags) to be presented to thymocytes. Self-Ags are either expressed by thymic stroma cells or transported to the thymus from the periphery by migratory dendritic cells (DCs); meanwhile, small blood-borne peptides can access the thymic parenchyma by diffusing across the vascular lining. Here we describe an additional pathway of thymic Ag acquisition that enables circulating antigenic macromolecules to access both murine and human thymi. This pathway depends on a subset of thymus-resident DCs, distinct from both parenchymal and circulating migratory DCs, that are positioned in immediate proximity to thymic microvessels where they extend cellular processes across the endothelial barrier into the blood stream. Transendothelial positioning of DCs depends on DC-expressed CX3CR1 and its endothelial ligand, CX3CL1, and disrupting this chemokine pathway prevents thymic acquisition of circulating proteins and compromises negative selection of Ag-reactive thymocytes. Thus, transendothelial DCs represent a mechanism by which the thymus can actively acquire blood-borne Ags to induce and maintain central tolerance.

Suggested Citation

  • Elisabeth H. Vollmann & Kristin Rattay & Olga Barreiro & Aude Thiriot & Rebecca A. Fuhlbrigge & Vladimir Vrbanac & Ki-Wook Kim & Steffen Jung & Andrew M. Tager & Ulrich H. von Andrian, 2021. "Specialized transendothelial dendritic cells mediate thymic T-cell selection against blood-borne macromolecules," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26446-x
    DOI: 10.1038/s41467-021-26446-x
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    References listed on IDEAS

    as
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    4. Jeremiah R. McDole & Leroy W. Wheeler & Keely G. McDonald & Baomei Wang & Vjollca Konjufca & Kathryn A. Knoop & Rodney D. Newberry & Mark J. Miller, 2012. "Goblet cells deliver luminal antigen to CD103+ dendritic cells in the small intestine," Nature, Nature, vol. 483(7389), pages 345-349, March.
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    Cited by:

    1. Hao Hu & Anthony N. Vomund & Orion J. Peterson & Neetu Srivastava & Tiandao Li & Lisa Kain & Wandy L. Beatty & Bo Zhang & Chyi-Song Hsieh & Luc Teyton & Cheryl F. Lichti & Emil R. Unanue & Xiaoxiao Wa, 2024. "Crinophagic granules in pancreatic β cells contribute to mouse autoimmune diabetes by diversifying pathogenic epitope repertoire," Nature Communications, Nature, vol. 15(1), pages 1-22, December.

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