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Directed Migration of Positively Selected Thymocytes Visualized in Real Time

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  • Colleen M Witt
  • Subhadip Raychaudhuri
  • Brian Schaefer
  • Arup K Chakraborty
  • Ellen A Robey

Abstract

Development of many vertebrate tissues involves long-range cell migrations. In most cases, these migrations have been inferred from analysis of single time points and the migration process has not been directly observed and quantitated in real time. In the mammalian adult thymus, immature CD4+CD8+ double-positive (DP) thymocytes are found in the outer cortex, whereas after T cell antigen receptor (TCR) repertoire selection, CD4+CD8– and CD4–CD8+ single-positive (SP) thymocytes are found in the central medulla. Here we have used two-photon laser-scanning microscopy and quantitative analysis of four-dimensional cell migration data to investigate the movement of thymocytes through the cortex in real time within intact thymic lobes. We show that prior to positive selection, cortical thymocytes exhibit random walk migration. In contrast, positive selection is correlated with the appearance of a thymocyte population displaying rapid, directed migration toward the medulla. These studies provide our first glimpse into the dynamics of developmentally programmed, long-range cell migration in the mammalian thymus. Two-photon laser-scanning microscopy reveals the change from random motion to directed migration that occurs when thymocytes undergo positive selection.

Suggested Citation

  • Colleen M Witt & Subhadip Raychaudhuri & Brian Schaefer & Arup K Chakraborty & Ellen A Robey, 2005. "Directed Migration of Positively Selected Thymocytes Visualized in Real Time," PLOS Biology, Public Library of Science, vol. 3(6), pages 1-1, May.
    • Handle: RePEc:plo:pbio00:0030160
    DOI: 10.1371/journal.pbio.0030160
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    References listed on IDEAS

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    1. Thorsten R. Mempel & Sarah E. Henrickson & Ulrich H. von Andrian, 2004. "T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases," Nature, Nature, vol. 427(6970), pages 154-159, January.
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    1. Daisuke Imoto & Nen Saito & Akihiko Nakajima & Gen Honda & Motohiko Ishida & Toyoko Sugita & Sayaka Ishihara & Koko Katagiri & Chika Okimura & Yoshiaki Iwadate & Satoshi Sawai, 2021. "Comparative mapping of crawling-cell morphodynamics in deep learning-based feature space," PLOS Computational Biology, Public Library of Science, vol. 17(8), pages 1-30, August.
    2. Edward J Banigan & Tajie H Harris & David A Christian & Christopher A Hunter & Andrea J Liu, 2015. "Heterogeneous CD8+ T Cell Migration in the Lymph Node in the Absence of Inflammation Revealed by Quantitative Migration Analysis," PLOS Computational Biology, Public Library of Science, vol. 11(2), pages 1-20, February.

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