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Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair

Author

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  • Maria Pilar Sanchez-Bailon

    (University of Birmingham
    Max Delbrück Center for Molecular Medicine)

  • Soo-Youn Choi

    (University of Birmingham)

  • Elizabeth R. Dufficy

    (University of Birmingham)

  • Karan Sharma

    (University of Birmingham)

  • Gavin S. McNee

    (University of Birmingham)

  • Emma Gunnell

    (University of Nottingham)

  • Kelly Chiang

    (University of Birmingham)

  • Debashish Sahay

    (University of Birmingham)

  • Sarah Maslen

    (MRC Laboratory of Molecular Biology)

  • Grant S. Stewart

    (University of Birmingham)

  • J. Mark Skehel

    (MRC Laboratory of Molecular Biology
    The Francis Crick Institute)

  • Ingrid Dreveny

    (University of Nottingham)

  • Clare C. Davies

    (University of Birmingham)

Abstract

Cross-talk between distinct protein post-translational modifications is critical for an effective DNA damage response. Arginine methylation plays an important role in maintaining genome stability, but how this modification integrates with other enzymatic activities is largely unknown. Here, we identify the deubiquitylating enzyme USP11 as a previously uncharacterised PRMT1 substrate, and demonstrate that the methylation of USP11 promotes DNA end-resection and the repair of DNA double strand breaks (DSB) by homologous recombination (HR), an event that is independent from another USP11-HR activity, the deubiquitylation of PALB2. We also show that PRMT1 is a ubiquitylated protein that it is targeted for deubiquitylation by USP11, which regulates the ability of PRMT1 to bind to and methylate MRE11. Taken together, our findings reveal a specific role for USP11 during the early stages of DSB repair, which is mediated through its ability to regulate the activity of the PRMT1-MRE11 pathway.

Suggested Citation

  • Maria Pilar Sanchez-Bailon & Soo-Youn Choi & Elizabeth R. Dufficy & Karan Sharma & Gavin S. McNee & Emma Gunnell & Kelly Chiang & Debashish Sahay & Sarah Maslen & Grant S. Stewart & J. Mark Skehel & I, 2021. "Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26413-6
    DOI: 10.1038/s41467-021-26413-6
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    References listed on IDEAS

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