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Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1

Author

Listed:
  • Valerie Garcia

    (Genome Damage and Stability Centre, The University of Sussex)

  • Sarah E. L. Phelps

    (Genome Damage and Stability Centre, The University of Sussex)

  • Stephen Gray

    (Genome Damage and Stability Centre, The University of Sussex)

  • Matthew J. Neale

    (Genome Damage and Stability Centre, The University of Sussex)

Abstract

DNA strand-break removal by Mre11 and Exo1 Specific DNA double-strand breaks are made during meiosis by Spo11, which remains bound to the DNA ends. Mre11 is a nuclease that can act exonucleolytically at DNA ends and endonucleolytically at internal sites. Previous studies have defined a role for the endonuclease, but not exonuclease, activity in DNA repair. Matthew Neale and colleagues show that Mre11 first makes a nick 300 bases from the end of the 5' strand, after which Mre11 degrades the DNA towards the break. Meanwhile, a second nuclease, Exo1, degrades the same strand in the opposite direction. This demonstrates that exonucleases can be loaded when the DNA end that is usually required for their initial binding is blocked.

Suggested Citation

  • Valerie Garcia & Sarah E. L. Phelps & Stephen Gray & Matthew J. Neale, 2011. "Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1," Nature, Nature, vol. 479(7372), pages 241-244, November.
    • Handle: RePEc:nat:nature:v:479:y:2011:i:7372:d:10.1038_nature10515
    DOI: 10.1038/nature10515
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    Cited by:

    1. Maria Pilar Sanchez-Bailon & Soo-Youn Choi & Elizabeth R. Dufficy & Karan Sharma & Gavin S. McNee & Emma Gunnell & Kelly Chiang & Debashish Sahay & Sarah Maslen & Grant S. Stewart & J. Mark Skehel & I, 2021. "Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
    2. Daipayan Banerjee & Kurt Langberg & Salar Abbas & Eric Odermatt & Praveen Yerramothu & Martin Volaric & Matthew A. Reidenbach & Kathy J. Krentz & C. Dustin Rubinstein & David L. Brautigan & Tarek Abba, 2021. "A non-canonical, interferon-independent signaling activity of cGAMP triggers DNA damage response signaling," Nature Communications, Nature, vol. 12(1), pages 1-24, December.

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