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Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time

Author

Listed:
  • Jan Andreas Ruland

    (University of Bonn)

  • Annika Marie Krüger

    (University of Bonn)

  • Kerstin Dörner

    (ETH Zurich)

  • Rohan Bhatia

    (University of Bonn)

  • Sabine Wirths

    (University of Bonn)

  • Daniel Poetes

    (University of Bonn)

  • Ulrike Kutay

    (ETH Zurich)

  • Jan Peter Siebrasse

    (University of Bonn)

  • Ulrich Kubitscheck

    (University of Bonn)

Abstract

Ribosomal biogenesis has been studied by biochemical, genetic and electron microscopic approaches, but live cell data on the in vivo kinetics are still missing. Here we analyse the export kinetics of the large ribosomal subunit (pre-60S particle) through single NPCs in human cells. We established a stable cell line co-expressing Halo-tagged eIF6 and GFP-fused NTF2 to simultaneously label pre-60S particles and NPCs, respectively. By combining single molecule tracking and super resolution confocal microscopy we visualize the dynamics of single pre-60S particles during export through single NPCs. For export events, maximum particle accumulation is found in the centre of the pore, while unsuccessful export terminates within the nuclear basket. The export has a single rate limiting step and a duration of ∼24 milliseconds. Only about 1/3 of attempted export events are successful. Our results show that the mass flux through a single NPC can reach up to ~125 MDa·s−1 in vivo.

Suggested Citation

  • Jan Andreas Ruland & Annika Marie Krüger & Kerstin Dörner & Rohan Bhatia & Sabine Wirths & Daniel Poetes & Ulrike Kutay & Jan Peter Siebrasse & Ulrich Kubitscheck, 2021. "Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26323-7
    DOI: 10.1038/s41467-021-26323-7
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    References listed on IDEAS

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    1. Alan R. Lowe & Jake J. Siegel & Petr Kalab & Merek Siu & Karsten Weis & Jan T. Liphardt, 2010. "Selectivity mechanism of the nuclear pore complex characterized by single cargo tracking," Nature, Nature, vol. 467(7315), pages 600-603, September.
    2. Franck Delavoie & Vanessa Soldan & Dana Rinaldi & Jean-Yves Dauxois & Pierre-Emmanuel Gleizes, 2019. "The path of pre-ribosomes through the nuclear pore complex revealed by electron tomography," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
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    5. Jens S. Andersen & Yun W. Lam & Anthony K. L. Leung & Shao-En Ong & Carol E. Lyon & Angus I. Lamond & Matthias Mann, 2005. "Nucleolar proteome dynamics," Nature, Nature, vol. 433(7021), pages 77-83, January.
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    Cited by:

    1. Sheung Chun Ng & Dirk Görlich, 2022. "A simple thermodynamic description of phase separation of Nup98 FG domains," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Sheung Chun Ng & Abin Biswas & Trevor Huyton & Jürgen Schünemann & Simone Reber & Dirk Görlich, 2023. "Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Martin F. Peter & Jan A. Ruland & Peer Depping & Niels Schneberger & Emmanuele Severi & Jonas Moecking & Karl Gatterdam & Sarah Tindall & Alexandre Durand & Veronika Heinz & Jan Peter Siebrasse & Paul, 2022. "Structural and mechanistic analysis of a tripartite ATP-independent periplasmic TRAP transporter," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    4. Martin F. Peter & Jan A. Ruland & Yeojin Kim & Philipp Hendricks & Niels Schneberger & Jan Peter Siebrasse & Gavin H. Thomas & Ulrich Kubitscheck & Gregor Hagelueken, 2024. "Conformational coupling of the sialic acid TRAP transporter HiSiaQM with its substrate binding protein HiSiaP," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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