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High-resolution three-dimensional mapping of mRNA export through the nuclear pore

Author

Listed:
  • Jiong Ma

    (Temple University)

  • Zhen Liu

    (Temple University)

  • Nicole Michelotti

    (Single Molecule Analysis Group, University of Michigan
    University of Michigan)

  • Sethuramasundaram Pitchiaya

    (Single Molecule Analysis Group, University of Michigan)

  • Ram Veerapaneni

    (Temple University)

  • John R. Androsavich

    (Single Molecule Analysis Group, University of Michigan
    Program in Chemical Biology, University of Michigan)

  • Nils G. Walter

    (Single Molecule Analysis Group, University of Michigan
    Program in Chemical Biology, University of Michigan)

  • Weidong Yang

    (Temple University)

Abstract

The flow of genetic information is regulated by selective nucleocytoplasmic transport of messenger RNA:protein complexes (mRNPs) through the nuclear pore complexes (NPCs) of eukaryotic cells. However, the three-dimensional (3D) pathway taken by mRNPs as they transit through the NPC, and the kinetics and selectivity of transport, remain obscure. Here we employ single-molecule fluorescence microscopy with an unprecedented spatiotemporal accuracy of 8 nm and 2 ms to provide new insights into the mechanism of nuclear mRNP export in live human cells. We find that mRNPs exiting the nucleus are decelerated and selected at the centre of the NPC, and adopt a fast-slow-fast diffusion pattern during their brief, ~12 ms, interaction with the NPC. A 3D reconstruction of the export route indicates that mRNPs primarily interact with the periphery on the nucleoplasmic side and in the centre of the NPC, without entering the central axial conduit utilized for passive diffusion of small molecules, and eventually dissociate on the cytoplasmic side.

Suggested Citation

  • Jiong Ma & Zhen Liu & Nicole Michelotti & Sethuramasundaram Pitchiaya & Ram Veerapaneni & John R. Androsavich & Nils G. Walter & Weidong Yang, 2013. "High-resolution three-dimensional mapping of mRNA export through the nuclear pore," Nature Communications, Nature, vol. 4(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3414
    DOI: 10.1038/ncomms3414
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    Cited by:

    1. Jan Andreas Ruland & Annika Marie Krüger & Kerstin Dörner & Rohan Bhatia & Sabine Wirths & Daniel Poetes & Ulrike Kutay & Jan Peter Siebrasse & Ulrich Kubitscheck, 2021. "Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Vesa Aho & Sami Salminen & Salla Mattola & Alka Gupta & Felix Flomm & Beate Sodeik & Jens B Bosse & Maija Vihinen-Ranta, 2021. "Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane," PLOS Pathogens, Public Library of Science, vol. 17(12), pages 1-20, December.

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