IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26168-0.html
   My bibliography  Save this article

Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology

Author

Listed:
  • Liang Zou

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Huihui Tian

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Shouliang Guan

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jianfei Ding

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Lei Gao

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jinfen Wang

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Ying Fang

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Optogenetics combined with electrical recording has emerged as a powerful tool for investigating causal relationships between neural circuit activity and function. However, the size of optogenetically manipulated tissue is typically 1-2 orders of magnitude larger than that can be electrically recorded, rendering difficulty for assigning functional roles of recorded neurons. Here we report a viral vector-delivery optrode (VVD-optrode) system for precise integration of optogenetics and electrophysiology in the brain. Our system consists of flexible microelectrode filaments and fiber optics that are simultaneously self-assembled in a nanoliter-scale, viral vector-delivery polymer carrier. The highly localized delivery and neuronal expression of opsin genes at microelectrode-tissue interfaces ensure high spatial congruence between optogenetically manipulated and electrically recorded neuronal populations. We demonstrate that this multifunctional system is capable of optogenetic manipulation and electrical recording of spatially defined neuronal populations for three months, allowing precise and long-term studies of neural circuit functions.

Suggested Citation

  • Liang Zou & Huihui Tian & Shouliang Guan & Jianfei Ding & Lei Gao & Jinfen Wang & Ying Fang, 2021. "Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26168-0
    DOI: 10.1038/s41467-021-26168-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26168-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26168-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Takao Someya & Zhenan Bao & George G. Malliaras, 2016. "The rise of plastic bioelectronics," Nature, Nature, vol. 540(7633), pages 379-385, December.
    2. James J. Jun & Nicholas A. Steinmetz & Joshua H. Siegle & Daniel J. Denman & Marius Bauza & Brian Barbarits & Albert K. Lee & Costas A. Anastassiou & Alexandru Andrei & Çağatay Aydın & Mladen Barbic &, 2017. "Fully integrated silicon probes for high-density recording of neural activity," Nature, Nature, vol. 551(7679), pages 232-236, November.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jongwoon Kim & Hengji Huang & Earl T. Gilbert & Kaiser C. Arndt & Daniel Fine English & Xiaoting Jia, 2024. "T-DOpE probes reveal sensitivity of hippocampal oscillations to cannabinoids in behaving mice," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yao Ni & Jiaqi Liu & Hong Han & Qianbo Yu & Lu Yang & Zhipeng Xu & Chengpeng Jiang & Lu Liu & Wentao Xu, 2024. "Visualized in-sensor computing," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    2. Guang Yao & Xiaoyi Mo & Shanshan Liu & Qian Wang & Maowen Xie & Wenhao Lou & Shiyan Chen & Taisong Pan & Ke Chen & Dezhong Yao & Yuan Lin, 2023. "Snowflake-inspired and blink-driven flexible piezoelectric contact lenses for effective corneal injury repair," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Edward A. B. Horrocks & Fabio R. Rodrigues & Aman B. Saleem, 2024. "Flexible neural population dynamics govern the speed and stability of sensory encoding in mouse visual cortex," Nature Communications, Nature, vol. 15(1), pages 1-23, December.
    4. Jérémie Sibille & Carolin Gehr & Jonathan I. Benichov & Hymavathy Balasubramanian & Kai Lun Teh & Tatiana Lupashina & Daniela Vallentin & Jens Kremkow, 2022. "High-density electrode recordings reveal strong and specific connections between retinal ganglion cells and midbrain neurons," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    5. Patrick Jendritza & Frederike J. Klein & Pascal Fries, 2023. "Multi-area recordings and optogenetics in the awake, behaving marmoset," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    6. Donghwan Ji & Jae Min Park & Myeong Seon Oh & Thanh Loc Nguyen & Hyunsu Shin & Jae Seong Kim & Dukjoon Kim & Ho Seok Park & Jaeyun Kim, 2022. "Superstrong, superstiff, and conductive alginate hydrogels," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    7. Yuzhong Hu & Kaushik Parida & Hao Zhang & Xin Wang & Yongxin Li & Xinran Zhou & Samuel Alexander Morris & Weng Heng Liew & Haomin Wang & Tao Li & Feng Jiang & Mingmin Yang & Marin Alexe & Zehui Du & C, 2022. "Bond engineering of molecular ferroelectrics renders soft and high-performance piezoelectric energy harvesting materials," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    8. Sakeena Saifi & Xiao Xiao & Simin Cheng & Haotian Guo & Jinsheng Zhang & Peter Müller-Buschbaum & Guangmin Zhou & Xiaomin Xu & Hui-Ming Cheng, 2024. "An ultraflexible energy harvesting-storage system for wearable applications," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    9. Morgan A. Brown & Kara M. Zappitelli & Loveprit Singh & Rachel C. Yuan & Melissa Bemrose & Valerie Brogden & David J. Miller & Matthew C. Smear & Stuart F. Cogan & Timothy J. Gardner, 2023. "Direct laser writing of 3D electrodes on flexible substrates," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    10. Peng Tan & Haifei Wang & Furui Xiao & Xi Lu & Wenhui Shang & Xiaobo Deng & Huafeng Song & Ziyao Xu & Junfeng Cao & Tiansheng Gan & Ben Wang & Xuechang Zhou, 2022. "Solution-processable, soft, self-adhesive, and conductive polymer composites for soft electronics," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    11. Longyu Ma & Lupeng Yue & Shuting Liu & Shi Xu & Jifu Tong & Xiaoyan Sun & Li Su & Shuang Cui & Feng-Yu Liu & You Wan & Ming Yi, 2024. "A distinct neuronal ensemble of prelimbic cortex mediates spontaneous pain in rats with peripheral inflammation," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    12. Bartul Mimica & Tuçe Tombaz & Claudia Battistin & Jingyi Guo Fuglstad & Benjamin A. Dunn & Jonathan R. Whitlock, 2023. "Behavioral decomposition reveals rich encoding structure employed across neocortex in rats," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    13. Yina Wei & Anirban Nandi & Xiaoxuan Jia & Joshua H. Siegle & Daniel Denman & Soo Yeun Lee & Anatoly Buchin & Werner Geit & Clayton P. Mosher & Shawn Olsen & Costas A. Anastassiou, 2023. "Associations between in vitro, in vivo and in silico cell classes in mouse primary visual cortex," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    14. Linda Bistere & Carlos M. Gomez-Guzman & Yirong Xiong & Daniela Vallentin, 2024. "Female calls promote song learning in male juvenile zebra finches," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    15. David Eriksson & Artur Schneider & Anupriya Thirumalai & Mansour Alyahyay & Brice Crompe & Kirti Sharma & Patrick Ruther & Ilka Diester, 2022. "Multichannel optogenetics combined with laminar recordings for ultra-controlled neuronal interrogation," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    16. Erik Hermansen & David A. Klindt & Benjamin A. Dunn, 2024. "Uncovering 2-D toroidal representations in grid cell ensemble activity during 1-D behavior," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    17. Spencer Ward & Conor Riley & Erin M. Carey & Jenny Nguyen & Sadik Esener & Axel Nimmerjahn & Donald J. Sirbuly, 2022. "Electro-optical mechanically flexible coaxial microprobes for minimally invasive interfacing with intrinsic neural circuits," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    18. Jun Li & Corey Carlos & Hao Zhou & Jiajie Sui & Yikai Wang & Zulmari Silva-Pedraza & Fan Yang & Yutao Dong & Ziyi Zhang & Timothy A. Hacker & Bo Liu & Yanchao Mao & Xudong Wang, 2023. "Stretchable piezoelectric biocrystal thin films," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    19. Keundong Lee & Angelique C. Paulk & Yun Goo Ro & Daniel R. Cleary & Karen J. Tonsfeldt & Yoav Kfir & John S. Pezaris & Youngbin Tchoe & Jihwan Lee & Andrew M. Bourhis & Ritwik Vatsyayan & Joel R. Mart, 2024. "Flexible, scalable, high channel count stereo-electrode for recording in the human brain," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    20. Ilke Uguz & David Ohayon & Volkan Arslan & Rajendar Sheelamanthula & Sophie Griggs & Adel Hama & John William Stanton & Iain McCulloch & Sahika Inal & Kenneth L. Shepard, 2024. "Flexible switch matrix addressable electrode arrays with organic electrochemical transistor and pn diode technology," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26168-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.