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Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology

Author

Listed:
  • Liang Zou

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Huihui Tian

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Shouliang Guan

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jianfei Ding

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Lei Gao

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Jinfen Wang

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology)

  • Ying Fang

    (CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
    Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Optogenetics combined with electrical recording has emerged as a powerful tool for investigating causal relationships between neural circuit activity and function. However, the size of optogenetically manipulated tissue is typically 1-2 orders of magnitude larger than that can be electrically recorded, rendering difficulty for assigning functional roles of recorded neurons. Here we report a viral vector-delivery optrode (VVD-optrode) system for precise integration of optogenetics and electrophysiology in the brain. Our system consists of flexible microelectrode filaments and fiber optics that are simultaneously self-assembled in a nanoliter-scale, viral vector-delivery polymer carrier. The highly localized delivery and neuronal expression of opsin genes at microelectrode-tissue interfaces ensure high spatial congruence between optogenetically manipulated and electrically recorded neuronal populations. We demonstrate that this multifunctional system is capable of optogenetic manipulation and electrical recording of spatially defined neuronal populations for three months, allowing precise and long-term studies of neural circuit functions.

Suggested Citation

  • Liang Zou & Huihui Tian & Shouliang Guan & Jianfei Ding & Lei Gao & Jinfen Wang & Ying Fang, 2021. "Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26168-0
    DOI: 10.1038/s41467-021-26168-0
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    References listed on IDEAS

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    1. Takao Someya & Zhenan Bao & George G. Malliaras, 2016. "The rise of plastic bioelectronics," Nature, Nature, vol. 540(7633), pages 379-385, December.
    2. James J. Jun & Nicholas A. Steinmetz & Joshua H. Siegle & Daniel J. Denman & Marius Bauza & Brian Barbarits & Albert K. Lee & Costas A. Anastassiou & Alexandru Andrei & Çağatay Aydın & Mladen Barbic &, 2017. "Fully integrated silicon probes for high-density recording of neural activity," Nature, Nature, vol. 551(7679), pages 232-236, November.
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    1. Jongwoon Kim & Hengji Huang & Earl T. Gilbert & Kaiser C. Arndt & Daniel Fine English & Xiaoting Jia, 2024. "T-DOpE probes reveal sensitivity of hippocampal oscillations to cannabinoids in behaving mice," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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