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Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region

Author

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  • Omar Nyabi

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

  • Mostafa Bentahir

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

  • Jérôme Ambroise

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

  • Bertrand Bearzatto

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

  • Nawfal Chibani

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

  • Benjamin Smits

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

  • Jean François Durant

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

  • Aleksandr Vybornov

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

  • Olivier Thellin

    (Centre de Recherche sur les Protéines Prions (CRPP) ULiège, Quartier Hôpital, 15, Avenue Hippocrate, B 4000 Liège, Belgium)

  • Benaissa El Moualij

    (Centre de Recherche sur les Protéines Prions (CRPP) ULiège, Quartier Hôpital, 15, Avenue Hippocrate, B 4000 Liège, Belgium)

  • Jean-Luc Gala

    (Center for Applied Molecular Technologies (CTMA), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1348 Woluwe saint-Lambert, Belgium)

Abstract

Coronavirus disease 2019 (COVID-19) is an acute infectious disease caused by the novel coronavirus (SARS-CoV-2) identified in 2019. The COVID-19 outbreak continues to have devastating consequences for human lives and the global economy. The B-LiFe mobile laboratory in Piedmont, Italy, was deployed for the surveillance of COVID-19 cases by large-scale testing of first responders. The objective was to assess the seroconversion among the regional civil protection (CP), police, health care professionals, and volunteers. The secondary objective was to detect asymptomatic individuals within this cohort in the light of age, sex, and residence. In this paper, we report the results of serological testing performed by the B-LiFe mobile laboratory deployed from 10 June to 23 July 2020. The tests included whole blood finger-prick and serum sampling for detection of SARS-CoV-2 spike receptor-binding domain (S-RBD) antibodies. The prevalence of SARS-CoV-2 antibodies was approximately 5% (294/6013). The results of the finger-prick tests and serum sample analyses showed moderate agreement (kappa = 0.77). Furthermore, the detection rates of serum antibodies to the SARS-CoV-2 nucleocapsid protein (NP) and S-RBD among the seroconverted individuals were positively correlated (kappa = 0.60), at least at the IgG level. Seroprevalence studies based on serological testing for the S-RBD protein or SARS-CoV-2 NP antibodies are not sufficient for diagnosis but might help in screening the population to be vaccinated and in determining the duration of seroconversion.

Suggested Citation

  • Omar Nyabi & Mostafa Bentahir & Jérôme Ambroise & Bertrand Bearzatto & Nawfal Chibani & Benjamin Smits & Jean François Durant & Aleksandr Vybornov & Olivier Thellin & Benaissa El Moualij & Jean-Luc Ga, 2021. "Diagnostic Value of IgM and IgG Detection in COVID-19 Diagnosis by the Mobile Laboratory B-LiFE: A Massive Testing Strategy in the Piedmont Region," IJERPH, MDPI, vol. 18(7), pages 1-10, March.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:7:p:3372-:d:523461
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    1. Aleksandr Vybornov & Omar Nyabi & Olga Vybornova & Jean-Luc Gala, 2021. "Telecommunication Facilities, Key Support for Data Management and Data Sharing by a Biological Mobile Laboratory Deployed to Counter Emerging Biological Threats and Improve Public Health Crisis Prepar," IJERPH, MDPI, vol. 18(17), pages 1-23, August.

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