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Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM 2.5 from Beijing, China, in Winter

Author

Listed:
  • Bing-Yu Niu

    (College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China)

  • Wen-Ke Li

    (College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China)

  • Jiang-Shuai Li

    (College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China)

  • Qi-Hao Hong

    (College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China)

  • Sara Khodahemmati

    (National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China)

  • Jing-Feng Gao

    (National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China)

  • Zhi-Xiang Zhou

    (College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China)

Abstract

Epidemiological studies have corroborated that respiratory diseases, including lung cancer, are related to fine particulate matter (<2.5 μm) (PM 2.5 ) exposure. The toxic responses of PM 2.5 are greatly influenced by the source of PM 2.5 . However, the effects of PM 2.5 from Beijing on bronchial genotoxicity are scarce. In the present study, PM 2.5 from Beijing was sampled and applied in vitro to investigate its genotoxicity and the mechanisms behind it. Human bronchial epithelial cells 16HBE were used as a model for exposure. Low (67.5 μg/mL), medium (116.9 μg/mL), and high (202.5 μg/mL) doses of PM 2.5 were used for cell exposure. After PM 2.5 exposure, cell viability, oxidative stress markers, DNA (deoxyribonucleic acid) strand breaks, 8-OH-dG levels, micronuclei formation, and DNA repair gene expression were measured. The results showed that PM 2.5 significantly induced cytotoxicity in 16HBE. Moreover, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and cellular heme oxygenase (HO-1) were increased, and the level of glutathione (GSH) was decreased, which represented the occurrence of severe oxidative stress in 16HBE. The micronucleus rate was elevated, and DNA damage occurred as indicators of the comet assay, γ-H2AX and 8-OH-dG, were markedly enhanced by PM 2.5 , accompanied by the influence of 8-oxoguanine DNA glycosylase (OGG1), X-ray repair cross-complementing gene 1 (XRCC1), and poly (ADP-ribose) polymerase-1 (PARP1) expression. These results support the significant role of PM 2.5 genotoxicity in 16HBE cells, which may occur through the combined effect on oxidative stress and the influence of DNA repair genes.

Suggested Citation

  • Bing-Yu Niu & Wen-Ke Li & Jiang-Shuai Li & Qi-Hao Hong & Sara Khodahemmati & Jing-Feng Gao & Zhi-Xiang Zhou, 2020. "Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM 2.5 from Beijing, China, in Winter," IJERPH, MDPI, vol. 17(13), pages 1-15, July.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:13:p:4874-:d:381034
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    References listed on IDEAS

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    1. Athanasios Valavanidis & Thomais Vlachogianni & Konstantinos Fiotakis & Spyridon Loridas, 2013. "Pulmonary Oxidative Stress, Inflammation and Cancer: Respirable Particulate Matter, Fibrous Dusts and Ozone as Major Causes of Lung Carcinogenesis through Reactive Oxygen Species Mechanisms," IJERPH, MDPI, vol. 10(9), pages 1-22, August.
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