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Effects of Inhibitory Compounds Present in Lignocellulosic Biomass Hydrolysates on the Growth of Bacillus subtilis

Author

Listed:
  • Lucas van der Maas

    (Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Building 223, 2800 Kongens Lyngby, Denmark
    The authors contributed equally to this work.)

  • Jasper L. S. P. Driessen

    (Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens Lyngby, Denmark
    The authors contributed equally to this work.)

  • Solange I. Mussatto

    (Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Building 223, 2800 Kongens Lyngby, Denmark)

Abstract

This study evaluated the individual and combined effects of inhibitory compounds formed during pretreatment of lignocellulosic biomass on the growth of Bacillus subtilis . Ten inhibitory compounds commonly present in lignocellulosic hydrolysates were evaluated, which included sugar degradation products (furfural and 5-hydroxymethylfurfural), acetic acid, and seven phenolic compounds derived from lignin (benzoic acid, vanillin, vanillic acid, ferulic acid, p -coumaric acid, 4-hydroxybenzoic acid, and syringaldehyde). For the individual inhibitors, syringaldehyde showed the most toxic effect, completely inhibiting the strain growth at 0.1 g/L. In the sequence, assays using mixtures of the inhibitory compounds at a concentration of 12.5% of their IC50 value were performed to evaluate the combined effect of the inhibitors on the strain growth. These experiments were planned according to a Plackett–Burman experimental design. Statistical analysis of the results revealed that in a mixture, benzoic acid and furfural were the most potent inhibitors affecting the growth of B. subtilis . These results contribute to a better understanding of the individual and combined effects of inhibitory compounds present in biomass hydrolysates on the microbial performance of B. subtilis . Such knowledge is important to advance the development of sustainable biomanufacturing processes using this strain cultivated in complex media produced from lignocellulosic biomass, supporting the development of efficient bio-based processes using B. subtilis .

Suggested Citation

  • Lucas van der Maas & Jasper L. S. P. Driessen & Solange I. Mussatto, 2021. "Effects of Inhibitory Compounds Present in Lignocellulosic Biomass Hydrolysates on the Growth of Bacillus subtilis," Energies, MDPI, vol. 14(24), pages 1-12, December.
    • Handle: RePEc:gam:jeners:v:14:y:2021:i:24:p:8419-:d:701753
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    References listed on IDEAS

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    1. Mussatto, Solange I. & Yamakawa, Celina K. & van der Maas, Lucas & Dragone, Giuliano, 2021. "New trends in bioprocesses for lignocellulosic biomass and CO2 utilization," Renewable and Sustainable Energy Reviews, Elsevier, vol. 152(C).
    2. Sivagurunathan, Periyasamy & Kumar, Gopalakrishnan & Mudhoo, Ackmez & Rene, Eldon R. & Saratale, Ganesh Dattatraya & Kobayashi, Takuro & Xu, Kaiqin & Kim, Sang-Hyoun & Kim, Dong-Hoon, 2017. "Fermentative hydrogen production using lignocellulose biomass: An overview of pre-treatment methods, inhibitor effects and detoxification experiences," Renewable and Sustainable Energy Reviews, Elsevier, vol. 77(C), pages 28-42.
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