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Cultivation Method Effect on Schizochytrium sp. Biomass Growth and Docosahexaenoic Acid (DHA) Production with the Use of Waste Glycerol as a Source of Organic Carbon

Author

Listed:
  • Natalia Kujawska

    (InnovaTree Sp. z o.o., 81-451 Gdynia, Poland)

  • Szymon Talbierz

    (InnovaTree Sp. z o.o., 81-451 Gdynia, Poland)

  • Marcin Dębowski

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland)

  • Joanna Kazimierowicz

    (Department of Water Supply and Sewage Systems, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, 15-351 Bialystok, Poland)

  • Marcin Zieliński

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland)

Abstract

Inexpensive carbon sources offering an alternative to glucose are searched for to reduce costs of docosahexaenoic acid production by microalgae. The use of waste glycerol seems substantiated and prospective in this case. The objective of this study was to determine the production yield of heterotrophic microalgae Schizochytrium sp. biomass and the efficiency of docosahexaenoic acid production in various types of cultures with waste glycerol. Cultivation conditions were optimized using the Plackett–Burman method and Response Surface Methodology. The highest technological performance was obtained in the fed-batch culture, where the concentration of Schizochytrium sp. biomass reached 103.44 ± 1.50 g/dm 3 , the lipid concentration in Schizochytrium sp. biomass was at 48.85 ± 0.81 g/dm 3 , and the docosahexaenoic acid concentration at 21.98 ± 0.36 g/dm 3 . The highest docosahexaenoic acid content, accounting for 61.76 ± 3.77% of total fatty acids, was determined in lipid bodies of the Schizochytrium sp. biomass produced in the batch culture, whereas the lowest one, accounting for 44.99 ± 2.12% of total fatty acids, in those of the biomass grown in the fed-batch culture.

Suggested Citation

  • Natalia Kujawska & Szymon Talbierz & Marcin Dębowski & Joanna Kazimierowicz & Marcin Zieliński, 2021. "Cultivation Method Effect on Schizochytrium sp. Biomass Growth and Docosahexaenoic Acid (DHA) Production with the Use of Waste Glycerol as a Source of Organic Carbon," Energies, MDPI, vol. 14(10), pages 1-16, May.
    • Handle: RePEc:gam:jeners:v:14:y:2021:i:10:p:2952-:d:558348
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    References listed on IDEAS

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    1. Natalia Kujawska & Szymon Talbierz & Marcin Dębowski & Joanna Kazimierowicz & Marcin Zieliński, 2021. "Optimizing Docosahexaenoic Acid (DHA) Production by Schizochytrium sp. Grown on Waste Glycerol," Energies, MDPI, vol. 14(6), pages 1-17, March.
    2. Okoro, Victor & Azimov, Ulugbek & Munoz, Jose & Hernandez, Hector H. & Phan, Anh N., 2019. "Microalgae cultivation and harvesting: Growth performance and use of flocculants - A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 115(C).
    3. Liu, Xiaoyan & Zhu, Fenfen & Zhang, Rongyan & Zhao, Luyao & Qi, Juanjuan, 2021. "Recent progress on biodiesel production from municipal sewage sludge," Renewable and Sustainable Energy Reviews, Elsevier, vol. 135(C).
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    1. Marcin Dębowski & Izabela Świca & Joanna Kazimierowicz & Marcin Zieliński, 2022. "Large Scale Microalgae Biofuel Technology—Development Perspectives in Light of the Barriers and Limitations," Energies, MDPI, vol. 16(1), pages 1-23, December.
    2. Marcin Zieliński & Marcin Dębowski & Joanna Kazimierowicz, 2022. "Outflow from a Biogas Plant as a Medium for Microalgae Biomass Cultivation—Pilot Scale Study and Technical Concept of a Large-Scale Installation," Energies, MDPI, vol. 15(8), pages 1-18, April.
    3. Marcin Zieliński & Marcin Dębowski & Joanna Kazimierowicz & Izabela Świca, 2023. "Microalgal Carbon Dioxide (CO 2 ) Capture and Utilization from the European Union Perspective," Energies, MDPI, vol. 16(3), pages 1-27, February.
    4. Szymon Talbierz & Marcin Dębowski & Natalia Kujawska & Joanna Kazimierowicz & Marcin Zieliński, 2022. "Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol," IJERPH, MDPI, vol. 19(5), pages 1-17, March.

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