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Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol

Author

Listed:
  • Szymon Talbierz

    (InnovaTree Sp. z o.o., 81-451 Gdynia, Poland)

  • Marcin Dębowski

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland)

  • Natalia Kujawska

    (InnovaTree Sp. z o.o., 81-451 Gdynia, Poland)

  • Joanna Kazimierowicz

    (Department of Water Supply and Sewage Systems, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, 15-351 Bialystok, Poland)

  • Marcin Zieliński

    (Department of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland)

Abstract

One of the most promising avenues of biofuel research relates to using waste as a starting feedstock to produce liquid or gaseous energy carriers. The global production of waste glycerol by the refinery industry is rising year after year. The aim of the present study was to examine the effect of ethyl methane sulfonate (EMS) on the growth rates and intracellular lipid accumulation in heterotrophically-cultured Schizochytrium limacinum microalgae, grown on waste glycerol as the carbon source. The strain S. limacinum E20, produced by incubating a reference strain in EMS for 20 min, was found to perform the best in terms of producing biomass (0.054 g DW /dm 3 ·h) and accumulating intracellular bio-oil (0.021 g/dm 3 ·h). The selected parameters proved to be optimal for S. limacinum E20 biomass growth at the following values: temperature 27.3 °C, glycerol level 249.0 g/dm 3 , oxygen in the culture 26%, and yeast extract concentration 45.0 g/dm 3 . In turn, the optimal values for lipid production in an S. limacinum E20 culture were: temperature 24.2 °C, glycerol level 223.0 g/dm 3 , oxygen in the culture 10%, and yeast extract concentration 10.0 g/dm 3 . As the process conditions are different for biomass growth and for intracellular lipid accumulation, it is recommended to use a two-step culture process, which resulted in a lipid synthesis rate of 0.41 g/dm 3 ·h.

Suggested Citation

  • Szymon Talbierz & Marcin Dębowski & Natalia Kujawska & Joanna Kazimierowicz & Marcin Zieliński, 2022. "Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol," IJERPH, MDPI, vol. 19(5), pages 1-17, March.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:5:p:3108-:d:765331
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    References listed on IDEAS

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    1. Natalia Kujawska & Szymon Talbierz & Marcin Dębowski & Joanna Kazimierowicz & Marcin Zieliński, 2021. "Cultivation Method Effect on Schizochytrium sp. Biomass Growth and Docosahexaenoic Acid (DHA) Production with the Use of Waste Glycerol as a Source of Organic Carbon," Energies, MDPI, vol. 14(10), pages 1-16, May.
    2. Mihaela Simionescu & Yuriy Bilan & Piotr Zawadzki & Adam Wojciechowski & Marcin Rabe, 2021. "GHG Emissions Mitigation in the European Union Based on Labor Market Changes," Energies, MDPI, vol. 14(2), pages 1-13, January.
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    Cited by:

    1. Joanna Kazimierowicz & Marcin Dębowski & Marcin Zieliński & Aneta Ignaciuk & Sandra Mlonek & Jordi Cruz Sanchez, 2024. "The Biosynthesis of Liquid Fuels and Other Value-Added Products Based on Waste Glycerol—A Comprehensive Review and Bibliometric Analysis," Energies, MDPI, vol. 17(12), pages 1-31, June.

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