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Pyramiding Submergence Tolerance and Three Bacterial Blight Resistance Genes in Popular Rice Variety Hasanta through Marker-Assisted Backcross Breeding

Author

Listed:
  • Goutam Das

    (Department of Plant Breeding and Genetics, Odisha Agricultural University and Technology, Bhubaneshwar 751003, India)

  • Banshidhar Pradhan

    (Department of Plant Breeding and Genetics, Odisha Agricultural University and Technology, Bhubaneshwar 751003, India)

  • Debendranath Bastia

    (Department of Plant Breeding and Genetics, Odisha Agricultural University and Technology, Bhubaneshwar 751003, India)

  • Sanghamitra Samantaray

    (ICAR-National Rice Research Institute, Cuttack 753006, India)

  • Debarchana Jena

    (ICAR-National Rice Research Institute, Cuttack 753006, India)

  • Diptibala Rout

    (ICAR-National Rice Research Institute, Cuttack 753006, India)

  • Paduranga Bhagwan Arsode

    (ICAR-National Rice Research Institute, Cuttack 753006, India)

  • Vineeta Singh

    (ICAR-National Rice Research Institute, Cuttack 753006, India)

  • Arup Kumar Mukherjee

    (ICAR-National Rice Research Institute, Cuttack 753006, India)

  • Chander Mohan

    (Department of Agriculture, Cooperation and Farmers Welfare, New Delhi-110001, India)

  • Ramlakhan Verma

    (ICAR-National Rice Research Institute, Cuttack 753006, India)

Abstract

Flash flooding (FF) along with bacterial blight (BB) outbreak are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem. The presence of dynamic Xoo races with varying levels of genetic diversity and virulence renders their management extremely challenging under RSL. In this context, the marker-assisted improvement of plant resistance/tolerance has been proven as one of the most promising strategies towards the development of sustainable cultivars. The present study demonstrates the marker-assisted introgression of the submergence tolerant ( Sub1 ) and three bacterial blight resistant genes ( Xa21 + xa13 + xa5 ) into the genetic background of Hasanta, a long duration popular rice variety in the eastern coastal region of India. The rice genotypes, Swarna Sub1 (carrying Sub1 ) and IRBB66 (carrying Xa21 + xa13 + Xa7 + xa5 + Xa4 ) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta; recurrent parent (RP) was used as donor. The forward analysis of target genes in F1s, IC1F1s and backcross (BC) generations was performed by linked/genic markers (Sub1bc2; pTA248, xa13prom and RM122), whereas background recovery of RP in each BC and segregating generations was performed utilizing 108 hypervariable SSR markers. Intervened speed breeding (SB) strategy and intensive phenotyping could lead the development of near isogenic lines (NILs) as to the RP in all basic traits. The performance of the near isogenic lines (NILs, BC 2 F 3 and BC 2 F 4 ), HS 232-411-391-756-37, HS 232-411-391-809-8, HS 232-411-391-756-18, HS 110-224-197-10-36, HS 232-411-391-809-81, HS 110-224-197-10-41 and HS 232-411-391-809-63 establishes the utility of marker-assisted backcross-breeding (MAB) and SB in accelerated trait introgression. The introgressed lines carrying Sub1 + Xa21 + xa13 + xa5 showed 76% to 91% survival under 14 days of submergence and durable BB resistance (percent disease index-PDI of 2.68 ± 0.26 to 6.22 ± 1.08 and lesion length, LL of 1.29 ± 0.12 to 4.2 ± 0.64 cm). Physiological analysis revealed that improved NILs, carrying Sub1 gene conquered adaptive physiological modulations, had reduced the consumption of soluble sugar and the degradation of total chlorophyll contents (TCC), and an enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes. The pyramided lines attained complete product profile of RP, that will contribute to sustainable rice production under RSL, particularly in the coastal region that has substantial acreage under the variety Hasanta.

Suggested Citation

  • Goutam Das & Banshidhar Pradhan & Debendranath Bastia & Sanghamitra Samantaray & Debarchana Jena & Diptibala Rout & Paduranga Bhagwan Arsode & Vineeta Singh & Arup Kumar Mukherjee & Chander Mohan & Ra, 2022. "Pyramiding Submergence Tolerance and Three Bacterial Blight Resistance Genes in Popular Rice Variety Hasanta through Marker-Assisted Backcross Breeding," Agriculture, MDPI, vol. 12(11), pages 1-26, October.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:11:p:1815-:d:959107
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    References listed on IDEAS

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    1. Kenong Xu & Xia Xu & Takeshi Fukao & Patrick Canlas & Reycel Maghirang-Rodriguez & Sigrid Heuer & Abdelbagi M. Ismail & Julia Bailey-Serres & Pamela C. Ronald & David J. Mackill, 2006. "Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice," Nature, Nature, vol. 442(7103), pages 705-708, August.
    2. Kartik Chandra Pradhan & Soumya Ranjan Barik & Shibani Mohapatra & Deepak Kumar Nayak & Elssa Pandit & Binod Kumar Jena & Sushree Sangeeta & Abhijit Pradhan & Abhishek Samal & Jitendiya Meher & Lambod, 2022. "Incorporation of Two Bacterial Blight Resistance Genes into the Popular Rice Variety, Ranidhan through Marker-Assisted Breeding," Agriculture, MDPI, vol. 12(9), pages 1-14, August.
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