IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v99y2016icp216-223.html
   My bibliography  Save this article

Biosynthesis of β-caryophyllene, a novel terpene-based high-density biofuel precursor, using engineered Escherichia coli

Author

Listed:
  • Yang, Jianming
  • Li, Zhengfeng
  • Guo, Lizhong
  • Du, Juan
  • Bae, Hyeun-Jong

Abstract

β-caryophyllene is a common sesquiterpene compound currently being studied as a promising precursor for the production of high-density fuels. Acute demand for high-density fuels has provided the impetus to pursue biosynthetic methods to produce β-caryophyllene from reproducible sources. In this study, we produced β-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain of which phosphoglucose isomerase gene has been deleted. The 1- deoxy-d-xylulose 5-phosphate (DXP) or heterologous mevalonate (MVA) pathways were employed. Meanwhile, geranyl diphosphate synthase, glucose-6-phosphate dehydrogenase and β-caryophyllene synthase genes were co-overexpressed in the above strain. The final genetically modified strain, YJM59, produced 220 ± 6 mg/L of β-caryophyllene in flask culture. We also evaluated the use of fed-batch fermentation for the production of β-caryophyllene. After induction for 60 h, the YJM59 strain produced β-caryophyllene at a concentration of 1520 mg/L. The volumetric production fermented in the aerobic fed-batch was 0.34 mg/(L·h·OD600) and the conversion efficiency of glucose to β-caryophyllene (gram to gram) was 1.69%. Our results are the first successful attempt to produce β-caryophyllene using E. coli BL21(DE3), and provide a new strategy that is green and sustainable for the production of β-caryophyllene.

Suggested Citation

  • Yang, Jianming & Li, Zhengfeng & Guo, Lizhong & Du, Juan & Bae, Hyeun-Jong, 2016. "Biosynthesis of β-caryophyllene, a novel terpene-based high-density biofuel precursor, using engineered Escherichia coli," Renewable Energy, Elsevier, vol. 99(C), pages 216-223.
  • Handle: RePEc:eee:renene:v:99:y:2016:i:c:p:216-223
    DOI: 10.1016/j.renene.2016.06.061
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148116305924
    Download Restriction: Full text for ScienceDirect subscribers only

    File URL: https://libkey.io/10.1016/j.renene.2016.06.061?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Eric J. Steen & Yisheng Kang & Gregory Bokinsky & Zhihao Hu & Andreas Schirmer & Amy McClure & Stephen B. del Cardayre & Jay D. Keasling, 2010. "Microbial production of fatty-acid-derived fuels and chemicals from plant biomass," Nature, Nature, vol. 463(7280), pages 559-562, January.
    2. Eva Chovancova & Antonin Pavelka & Petr Benes & Ondrej Strnad & Jan Brezovsky & Barbora Kozlikova & Artur Gora & Vilem Sustr & Martin Klvana & Petr Medek & Lada Biedermannova & Jiri Sochor & Jiri Damb, 2012. "CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures," PLOS Computational Biology, Public Library of Science, vol. 8(10), pages 1-12, October.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Keri A McKiernan & Anna K Koster & Merritt Maduke & Vijay S Pande, 2020. "Dynamical model of the CLC-2 ion channel reveals conformational changes associated with selectivity-filter gating," PLOS Computational Biology, Public Library of Science, vol. 16(3), pages 1-24, March.
    2. Fernand, Francois & Israel, Alvaro & Skjermo, Jorunn & Wichard, Thomas & Timmermans, Klaas R. & Golberg, Alexander, 2017. "Offshore macroalgae biomass for bioenergy production: Environmental aspects, technological achievements and challenges," Renewable and Sustainable Energy Reviews, Elsevier, vol. 75(C), pages 35-45.
    3. Valle-Rodríguez, Juan Octavio & Shi, Shuobo & Siewers, Verena & Nielsen, Jens, 2014. "Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways," Applied Energy, Elsevier, vol. 115(C), pages 226-232.
    4. Das, Manali & Patra, Pradipta & Ghosh, Amit, 2020. "Metabolic engineering for enhancing microbial biosynthesis of advanced biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 119(C).
    5. Mhatre, Apurv & Kalscheur, Bethany & Mckeown, Haley & Bhakta, Karan & Sarnaik, Aditya P. & Flores, Andrew & Nielsen, David R. & Wang, Xuan & Soundappan, Thiagarajan & Varman, Arul M., 2022. "Consolidated bioprocessing of hemicellulose to fuels and chemicals through an engineered Bacillus subtilis-Escherichia coli consortium," Renewable Energy, Elsevier, vol. 193(C), pages 288-298.
    6. Bhatia, Shashi Kant & Bhatia, Ravi Kant & Yang, Yung-Hun, 2017. "An overview of microdiesel — A sustainable future source of renewable energy," Renewable and Sustainable Energy Reviews, Elsevier, vol. 79(C), pages 1078-1090.
    7. Oncel, Suphi S., 2013. "Microalgae for a macroenergy world," Renewable and Sustainable Energy Reviews, Elsevier, vol. 26(C), pages 241-264.
    8. Li, Mantian & Chen, Jinyi & Huang, Youjie & Li, Meichen & Lin, Xiaocheng & Qiu, Ting, 2020. "Reusable and efficient heterogeneous catalysts for biodiesel production from free fatty acids and oils: Self-solidifying hybrid ionic liquids," Energy, Elsevier, vol. 211(C).
    9. Chen, Hong-Ge & Zhang, Y.-H. Percival, 2015. "New biorefineries and sustainable agriculture: Increased food, biofuels, and ecosystem security," Renewable and Sustainable Energy Reviews, Elsevier, vol. 47(C), pages 117-132.
    10. Sadhukhan, Jhuma & Lloyd, Jon R. & Scott, Keith & Premier, Giuliano C. & Yu, Eileen H. & Curtis, Tom & Head, Ian M., 2016. "A critical review of integration analysis of microbial electrosynthesis (MES) systems with waste biorefineries for the production of biofuel and chemical from reuse of CO2," Renewable and Sustainable Energy Reviews, Elsevier, vol. 56(C), pages 116-132.
    11. Srirangan, Kajan & Akawi, Lamees & Moo-Young, Murray & Chou, C. Perry, 2012. "Towards sustainable production of clean energy carriers from biomass resources," Applied Energy, Elsevier, vol. 100(C), pages 172-186.
    12. Graham Rockwell & Nicholas J Guido & George M Church, 2013. "Redirector: Designing Cell Factories by Reconstructing the Metabolic Objective," PLOS Computational Biology, Public Library of Science, vol. 9(1), pages 1-15, January.
    13. Wang, Wei-Cheng & Tao, Ling, 2016. "Bio-jet fuel conversion technologies," Renewable and Sustainable Energy Reviews, Elsevier, vol. 53(C), pages 801-822.
    14. Lim, Jackson Hwa Keen & Gan, Yong Yang & Ong, Hwai Chyuan & Lau, Beng Fye & Chen, Wei-Hsin & Chong, Cheng Tung & Ling, Tau Chuan & Klemeš, Jiří Jaromír, 2021. "Utilization of microalgae for bio-jet fuel production in the aviation sector: Challenges and perspective," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).
    15. Bwapwa, Joseph K. & Anandraj, Akash & Trois, Cristina, 2017. "Possibilities for conversion of microalgae oil into aviation fuel: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 80(C), pages 1345-1354.
    16. Zhaoshou Wang & Xin Wu & Jianghai Peng & Yidan Hu & Baishan Fang & Shiyang Huang, 2014. "Artificially Constructed Quorum-Sensing Circuits Are Used for Subtle Control of Bacterial Population Density," PLOS ONE, Public Library of Science, vol. 9(8), pages 1-7, August.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:99:y:2016:i:c:p:216-223. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.