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Influence of inoculum on process parameters and microbial communities during anaerobic digestion of cattle manure: Insights from metabarcoding analysis

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  • Niya, Btissam
  • Azaroual, Salah Eddine
  • Kaichouh, Salma
  • Sendide, Khalid
  • Yaakoubi, Kaoutar
  • Fal, Soufiane
  • Rabia, Reda
  • Beraich, Fatima Zahra
  • Arouch, Moha
  • Meftah Kadmiri, Issam

Abstract

Linking the variety of microbial ecology attributes to system functioning is critical to improve operational strategies and to support microbial pathways, in order to conceive efficient and reliable anaerobic digestion process. In the present study, we conducted a direct comparison between two digesters regarding the process parameters and microbial community dynamics between two digesters: one containing only the substrate (cattle manure) (Digester S), and the other (Digester F) enriched with an inoculum. This study aimed to investigate taxonomic and functional changes in the digester microbiome using a 16S rRNA sequencing approach. Results exhibit that the bacterial richness, diversity, and regularity were higher in Digester F. This later showed also a higher biogas yields (BY) and the process performance indicators compared to Digester S. The BY obtained in digester F was 2.17 times higher than that obtained in digester S. Furthermore, significant correlations were obtained between biogas yields (BY) and microbial communities with Firmicutes/Bacillota (Clostridium, Enterococcus, Streptococcus, Ruminococcaceae, Mogibacterium, and Turicibacter), Actinobacteria (Corynebacterium), and Bacteroidetes (Bacteroides, Paludibacter) in the AD systems. Conversely, in Digester S, biogas yield (BY) was highly significant and positively correlated with the abundance of Actinobacteria (Rhodococcus) and Proteobacteria (Moraxella, Oxalobacter). Moreover, the inoculum in digester F resulted in the enrichment of main bacterial functions involved in biogas production. This study provides results that justify the changes in the structure of the microbial community and the functional strategy used by the most representative micro-organisms in the consortia that carried out this process.

Suggested Citation

  • Niya, Btissam & Azaroual, Salah Eddine & Kaichouh, Salma & Sendide, Khalid & Yaakoubi, Kaoutar & Fal, Soufiane & Rabia, Reda & Beraich, Fatima Zahra & Arouch, Moha & Meftah Kadmiri, Issam, 2024. "Influence of inoculum on process parameters and microbial communities during anaerobic digestion of cattle manure: Insights from metabarcoding analysis," Renewable Energy, Elsevier, vol. 231(C).
    • Handle: RePEc:eee:renene:v:231:y:2024:i:c:s0960148124010279
    DOI: 10.1016/j.renene.2024.120959
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    References listed on IDEAS

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    1. Magdalena Lebiocka & Agnieszka Montusiewicz & Agnieszka Cydzik-Kwiatkowska, 2018. "Effect of Bioaugmentation on Biogas Yields and Kinetics in Anaerobic Digestion of Sewage Sludge," IJERPH, MDPI, vol. 15(8), pages 1-16, August.
    2. Mao, Chunlan & Wang, Xiaojiao & Xi, Jianchao & Feng, Yongzhong & Ren, Guangxin, 2017. "Linkage of kinetic parameters with process parameters and operational conditions during anaerobic digestion," Energy, Elsevier, vol. 135(C), pages 352-360.
    3. Zhang, Jingxin & Loh, Kai-Chee & Li, Wangliang & Lim, Jun Wei & Dai, Yanjun & Tong, Yen Wah, 2017. "Three-stage anaerobic digester for food waste," Applied Energy, Elsevier, vol. 194(C), pages 287-295.
    4. Btissam Niya & Kaoutar Yaakoubi & Salah Eddine Azaroual & Fatima Zahra Beraich & Moha Arouch & Issam Meftah Kadmiri, 2023. "Anaerobic Digestion of Agricultural Waste Using Microbial Inocula: Performance and Characterization of Bacterial Communities Using 16S rRNA Sequencing Approach," Energies, MDPI, vol. 16(8), pages 1-15, April.
    5. Shen, Ruixia & Geng, Tao & Yao, Zonglu & Yu, Jiadong & Luo, Juan & Wang, Hongliang & Zhao, Lixin, 2023. "Characteristics of instability and suitable early-warning indicators for cornstalk-fed anaerobic digestion subjected to various sudden changes," Energy, Elsevier, vol. 278(C).
    6. Tedesco, S. & Daniels, S., 2019. "Evaluation of inoculum acclimatation and biochemical seasonal variation for the production of renewable gaseous fuel from biorefined Laminaria sp. waste streams," Renewable Energy, Elsevier, vol. 139(C), pages 1-8.
    7. Zamri, M.F.M.A. & Hasmady, Saiful & Akhiar, Afifi & Ideris, Fazril & Shamsuddin, A.H. & Mofijur, M. & Fattah, I. M. Rizwanul & Mahlia, T.M.I., 2021. "A comprehensive review on anaerobic digestion of organic fraction of municipal solid waste," Renewable and Sustainable Energy Reviews, Elsevier, vol. 137(C).
    8. Pengjiao Tian & Binbin Gong & Kaijian Bi & Yuxin Liu & Jing Ma & Xiqing Wang & Zhangsun Ouyang & Xian Cui, 2023. "Anaerobic Co-Digestion of Pig Manure and Rice Straw: Optimization of Process Parameters for Enhancing Biogas Production and System Stability," IJERPH, MDPI, vol. 20(1), pages 1-14, January.
    9. Westerholm, M. & Isaksson, S. & Karlsson Lindsjö, O. & Schnürer, A., 2018. "Microbial community adaptability to altered temperature conditions determines the potential for process optimisation in biogas production," Applied Energy, Elsevier, vol. 226(C), pages 838-848.
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