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Ethanol production from olive stones using different process strategies

Author

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  • Romero-García, J.M.
  • Susmozas, A.
  • Padilla-Rascón, C.
  • Manzanares, P.
  • Castro, E.
  • Oliva, J.M.
  • Romero, I.

Abstract

In this work, olive stone is used as a lignocellulosic raw material for ethanol production. In order to optimise the ethanol production yield, three different process strategies are studied considering the different streams produced in a sequential pretreatment of olive stone with dilute sulfuric acid/steam explosion (SE), which has been previously tested and demonstrated to be an effective fractionation strategy for olive stone biomass. Strategy 1 features fermentation with E. coli SL100 of the mixture of the detoxified prehydrolysate from the dilute sulfuric acid stage and the enzymatic hydrolysate of WIS and detoxified SE liquid fractions. Strategy 2 consists of fermentation with E. coli SL100 separately from the prehydrolysate and the enzymatic hydrolysate of WIS and detoxified SE liquid fractions. Strategy 3 considers fermentation with E. coli SL100 of the prehydrolysate from the acid stage and presaccharification and simultaneous saccharification and fermentation with S. cerevisiae “Ethanol Red” of WIS and detoxified SE liquid fractions. Strategies 2 and 3 reach a similar ethanol production of ∼162 kg/t, which is the highest ethanol yield reported so far from olive stones. The latter strategy uses two different microorganisms that allow an ethanol concentration close to 30 g/L.

Suggested Citation

  • Romero-García, J.M. & Susmozas, A. & Padilla-Rascón, C. & Manzanares, P. & Castro, E. & Oliva, J.M. & Romero, I., 2022. "Ethanol production from olive stones using different process strategies," Renewable Energy, Elsevier, vol. 194(C), pages 1174-1183.
    • Handle: RePEc:eee:renene:v:194:y:2022:i:c:p:1174-1183
    DOI: 10.1016/j.renene.2022.06.022
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    References listed on IDEAS

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    1. Pablo Doménech & Aleta Duque & Isabel Higueras & Raquel Iglesias & Paloma Manzanares, 2020. "Biorefinery of the Olive Tree—Production of Sugars from Enzymatic Hydrolysis of Olive Stone Pretreated by Alkaline Extrusion," Energies, MDPI, vol. 13(17), pages 1-13, September.
    2. Castro, Eulogio & Nieves, Ismael U. & Mullinnix, Mike T. & Sagues, William J. & Hoffman, Ralph W. & Fernández-Sandoval, Marco T. & Tian, Zhuoli & Rockwood, Donald L. & Tamang, Bijay & Ingram, Lonnie O, 2014. "Optimization of dilute-phosphoric-acid steam pretreatment of Eucalyptus benthamii for biofuel production," Applied Energy, Elsevier, vol. 125(C), pages 76-83.
    3. Manzanares, P. & Ballesteros, I. & Negro, M.J. & González, A. & Oliva, J.M. & Ballesteros, M., 2020. "Processing of extracted olive oil pomace residue by hydrothermal or dilute acid pretreatment and enzymatic hydrolysis in a biorefinery context," Renewable Energy, Elsevier, vol. 145(C), pages 1235-1245.
    4. Cuevas, Manuel & Sánchez, Sebastián & García, Juan F. & Baeza, Jaime & Parra, Carolina & Freer, Juanita, 2015. "Enhanced ethanol production by simultaneous saccharification and fermentation of pretreated olive stones," Renewable Energy, Elsevier, vol. 74(C), pages 839-847.
    5. Maria José Negro & Cristina Álvarez & Pablo Doménech & Raquel Iglesias & Ignacio Ballesteros, 2020. "Sugars Production from Municipal Forestry and Greening Wastes Pretreated by an Integrated Steam Explosion-Based Process," Energies, MDPI, vol. 13(17), pages 1-14, August.
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    1. Castro, Eulogio & Rabelo, Camila A.B. Silva & Padilla-Rascón, Carmen & Vidal, Alfonso M. & López-Linares, Juan C. & Varesche, Maria Bernadete A. & Romero, Inmaculada, 2023. "Biological hydrogen and furfural production from steam-exploded vine shoots," Renewable Energy, Elsevier, vol. 219(P1).
    2. Xie, Xinyu & Song, Kai & Wang, Jing & Hu, Jinguang & Wu, Shufang & Chu, Qiulu, 2024. "Efficient ethanol production from masson pine sawdust by various organosolv pretreatment and modified pre-hydrolysis simultaneous saccharification and fermentation," Renewable Energy, Elsevier, vol. 225(C).

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