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Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis

Author

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  • Paz-Cedeno, Fernando Roberto
  • Carceller, Jose Miguel
  • Iborra, Sara
  • Donato, Ricardo Keitel
  • Godoy, Anna Paula
  • Veloso de Paula, Ariela
  • Monti, Rubens
  • Corma, Avelino
  • Masarin, Fernando

Abstract

For producing second-generation ethanol (cellulosic ethanol) and other value-added bioproducts, magnetic graphene oxide (GO-MNP) was synthesized in this work and used as the immobilization support for an industrial cellulase-and xylanase-containing preparation. GO-MNP characterization by TEM, SEM and ATR-FTIR spectroscopy showed that the magnetic nanoparticles are homogeneously distributed onto the GO sheets surface. The enzymatic preparation was immobilized by means of carbodiimide cross-linking chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (NHS). The supported final biocatalyst (GO-MNP-Enz) showed high activity for the hydrolysis of pretreated sugarcane bagasse (PSB) and presented relative endoglucanase, xylanase, β-glucosidase, and β-xylosidase activities of 70%, 66%, 88%, and 70%, respectively, after 10 cycles of hydrolysis of their respective substrates. The biocatalyst also maintained approximately 50% and 80% of its efficiency for cellulose and xylan hydrolysis, respectively, being the TOF (g.g−1.h−1) the highest observed when compared with previous results reported in literature. These findings suggest that GO-MNP-Enz may be a prospective candidate for industrial applications such as second-generation ethanol production.

Suggested Citation

  • Paz-Cedeno, Fernando Roberto & Carceller, Jose Miguel & Iborra, Sara & Donato, Ricardo Keitel & Godoy, Anna Paula & Veloso de Paula, Ariela & Monti, Rubens & Corma, Avelino & Masarin, Fernando, 2021. "Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis," Renewable Energy, Elsevier, vol. 164(C), pages 491-501.
    • Handle: RePEc:eee:renene:v:164:y:2021:i:c:p:491-501
    DOI: 10.1016/j.renene.2020.09.059
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    Cited by:

    1. Guilherme, Ederson Paulo Xavier & Zanphorlin, Leticia Maria & Sousa, Amanda Silva & Miyamoto, Renan Yuji & Bruziquesi, Carlos Giovani Oliveira & Mesquita, Bruna Mara Aparecida de Carvalho & Santos, Se, 2022. "Simultaneous saccharification isomerization and Co-fermentation – SSICF: A new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO Sacchar," Renewable Energy, Elsevier, vol. 182(C), pages 274-284.

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