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Ultrasound-assisted enzymatic preparation of fatty acid ethyl ester in deep eutectic solvent

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  • Hao, Xiaohong
  • Suo, Hang
  • Zhang, Guanhua
  • Xu, Peixing
  • Gao, Xin
  • Du, Su

Abstract

In this paper, fatty acid ethyl ester (FAEE) was enzymatically produced from the waste oil and ethanol. It was investigated under three different conditions: ultrasound, deep eutectic solvent (DES) and ultrasound-assisted DES. The effects of lipase dosage, alcohol oil ratio, reaction temperature, ultrasonic power, reaction time and DES dosage on the conversion rate of FAEE were explored. The ultrasonic-assisted DES optimization experiment was carried out through the response surface experiment with five factors and three levels. The experimental results showed that the conversion of FAEE was significantly improved and the reaction time was shortened under the ultrasound-assisted DES system, the ultrasonic power and reaction temperature had synergistic effect on the conversion of FAEE. The conversion rate of FAEE reached 93.61 ± 0.46% on the condition of reaction temperature 59 °C, ultrasonic power 80 W, and reaction time 137 min with addition of 6.4% lipase and 4 mmol alcohol for 1 g oil.

Suggested Citation

  • Hao, Xiaohong & Suo, Hang & Zhang, Guanhua & Xu, Peixing & Gao, Xin & Du, Su, 2021. "Ultrasound-assisted enzymatic preparation of fatty acid ethyl ester in deep eutectic solvent," Renewable Energy, Elsevier, vol. 164(C), pages 937-947.
    • Handle: RePEc:eee:renene:v:164:y:2021:i:c:p:937-947
    DOI: 10.1016/j.renene.2020.09.114
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    References listed on IDEAS

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    1. Goli, Jibril & Sahu, Omprakash, 2018. "Development of heterogeneous alkali catalyst from waste chicken eggshell for biodiesel production," Renewable Energy, Elsevier, vol. 128(PA), pages 142-154.
    2. Williamson, Scott T. & Shahbaz, Kaveh & Mjalli, Farouq S. & AlNashef, Inas M. & Farid, Mohammed M., 2017. "Application of deep eutectic solvents as catalysts for the esterification of oleic acid with glycerol," Renewable Energy, Elsevier, vol. 114(PB), pages 480-488.
    3. Zhang, Huaxia & Liu, Tianshu & Zhu, Yawei & Hong, Li & Li, Tianfeng & Wang, Xiqing & Fu, Yujie, 2020. "Lipases immobilized on the modified polyporous magnetic cellulose support as an efficient and recyclable catalyst for biodiesel production from Yellow horn seed oil," Renewable Energy, Elsevier, vol. 145(C), pages 1246-1254.
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